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P rbs807 s811

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P-RbS807/S811 is a phospho-specific antibody that recognizes the phosphorylated serine 807 and serine 811 residues on the retinoblastoma protein (Rb). This antibody is used to detect the phosphorylation state of Rb, which is an important regulatory protein in cell cycle control.

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Lab products found in correlation

γh2ax, by Merck Group (2 mentions) P cjuns73, by Cell Signaling Technology (2 mentions) Goat anti rabbit igg alexa 488 conjugate, by Thermo Fisher Scientific (2 mentions) Fluorophore conjugated antibodies, by Cell Signaling Technology (2 mentions) C jun, by Cell Signaling Technology (2 mentions) Rpa32, by Abcam (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Dna pkcs, by Cell Signaling Technology (1 mentions) Ph2ax s139 clone jbw301, by Merck Group (1 mentions) Pdna pkcs s2056, by Cell Signaling Technology (1 mentions) Geminin, by Cell Signaling Technology (1 mentions) Prpa32 s4 s8, by Fortis Life Sciences (1 mentions)

3 protocols using p rbs807 s811

1

Comprehensive Antibody Characterization for CycIF

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The specific animal sources, catalogue numbers and dilutions for fluorophore-conjugated antibodies from Cell Signaling Technology are listed in Supplementary Table 1. The source and dilution of un-conjugated antibodies used in this study are as follows: p-RbS807/S811 (rabbit mAb; Cell Signaling:#8516 1:1000); p-cJunS73 (rabbit mAb; Cell Signaling:#3270; 1:500); c-Jun (rabbit mAb, Cell Signaling:#9165; 1:500); p53 (mouse mAb, Santa Cruz Biotech. sc-126, 1:200); γH2A.X (mouse mAb, Millipore: 05-636, 1:1000); Goat-anti-Rabbit IgG, Alexa-488 conjugate (Invitrogen:A-11034, 1:2000); Goat-anti-Mouse IgG, Alexa 647 conjugate (Invitrogen:A-21235, 1:2000). In Supplementary table 3, we listed all the antibodies we have already tested in CycIF protocol, including antibodies from several different vendors (Cell Signaling Technology, Abcam, Santa Cruz Technology & Biolegend). More updates will be available in the HMS-LINCS website (http://lincs.hms.harvard.edu/lin-NatCommun-2015/).
Lin J.R., Fallahi-Sichani M, & Sorger P.K. (2015). Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method. Nature Communications, 6, 8390.
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2

Multicolor Immunofluorescence Staining Protocol

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The specific animal sources, catalogue numbers and dilutions for fluorophore-conjugated antibodies from Cell Signaling Technology are listed in Supplementary Table 1. The source and dilution of unconjugated antibodies used in this study are as follows: p-RbS807/S811 (rabbit mAb; Cell Signaling:#8516 1:1,000); p-cJunS73 (rabbit mAb; Cell Signaling:#3270; 1:500 ); c-Jun (rabbit mAb, Cell Signaling:#9165; 1:500); p53 (mouse mAb, Santa Cruz Biotech. sc-126, 1:200); γH2A.X (mouse mAb, Millipore: 05-636, 1:1,000); Goat-anti-Rabbit IgG, Alexa-488 conjugate (Invitrogen:A-11034, 1:2,000); Goat-anti-Mouse IgG, Alexa 647 conjugate (Invitrogen:A-21235, 1:2,000). In Supplementary Table 3, we listed all the antibodies we have already tested in CycIF protocol, including antibodies from several different vendors (Cell Signaling Technology, Abcam, Santa Cruz Technology and Biolegend). More updates will be available in the HMS-LINCS website (http://lincs.hms.harvard.edu/lin-NatCommun-2015/).
Lin J.R., Fallahi-Sichani M, & Sorger P.K. (2015). Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method. Nature Communications, 6, 8390.
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3

Antibody Assay for Cell Cycle Markers

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Antibodies against pChk1 (S296), Cdc6, E2F1, RPA32 and RRM2, and were purchased from Abcam; pCDK1/2 (Y15), CDT1, pChk1 (S317), Chk1, pChk2 (T68), Chk2, Cyclin A2, Cyclin E1, pDNA-PKcs (S2056), DNA-PKcs, GAPDH, Geminin, pH2AX (S139), pHH3 (S10), Ki67, MCM2, PCNA, pRb (S807/S811) and Rb from Cell Signaling Technologies; pRPA32 (S4/S8) from Bethyl Laboratories and pH2AX (S139) (clone JBW301) from Merck Millipore. Antibodies were used at the manufacturer’s recommended dilutions.
, & Massey A.J. (2016). Tumour growth environment modulates Chk1 signalling pathways and Chk1 inhibitor sensitivity. Scientific Reports, 6, 35874.
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