At the peak stage (18–20 days) of behavioral symptoms following EAE induction, the lumbar segments of the spinal cords (n = 3 per group) were cropped. Immunoblot analysis was accomplished as previously described [35 (link),37 (link)] using mouse anti-platelet endothelial cell adhesion molecule [PECAM-1 (CD31); 1: 500; Santa Cruz biotechnology, Santa Cruz, CA, USA)]. Rabbit anti-fibronectin (1: 500; Santa Cruz biotechnology), Rabbit anti-MMP-9 (1:500, Abcam, Cambridge, UK), rabbit anti-p-ERK/p-JNK/p-P38 (1:1,000; Cell Signaling Technology, Beverly, MA, USA) antibodies, and HRP-conjugated secondary antibodies (1:200; Vector Laboratories, Burlingame, CA, USA). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:1,000; Cell Signaling Technology) was used as normalization for relative protein quantification.
Rabbit anti p erk p jnk p p38
Manufactured by Cell Signaling Technology
Sourced in United Kingdom, United States
Rabbit anti-p-ERK/p-JNK/p-P38 is a lab equipment product that detects phosphorylated forms of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (p38 MAPK) in various sample types using immunodetection methods.
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Lab products found in correlation
Rabbit anti mmp 9, by Abcam (1 mentions)
Pecam 1 cd31, by Santa Cruz Biotechnology (1 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Cell Signaling Technology (1 mentions)
Hrp conjugated secondary antibody, by Vector Laboratories (1 mentions)
Rabbit anti fibronectin, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Goat anti il 10, by R&D Systems (1 mentions)
Rabbit anti p iκbα, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rabbit anti p erk p jnk p p38
1
Spinal Cord Protein Expression in EAE
2
Western Blot Analysis of EAE Spinal Cords
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At the peak stage (14–16 days) of clinical score after induction of EAE, the lumbar segments of the spinal cords were harvested. Western blot analysis was performed as previously described (Jang and Cho, 2016 (link); Lee et al., 2016a (link)) using rabbit anti-p-IκBα (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-NF-κB p65 (1:1,000; Santa Cruz Biotechnology), rabbit anti-p-ERK/p-JNK/p-P38 (1:1,000; Cell Signaling Technology, Beverly, MA, USA), rabbit anti-ERK/JNK/P38 (1:1,000; Cell Signaling Technology), goat anti-IL-10 (1:500, R&D systems, Minneapolis, MN, USA), rabbit anti-inducible nitric oxide synthases (iNOS) (1:500; sigma), and mouse anti-cyclooxygenase-2 (COX-2) (1:500, BD Biosciences, San Joes, CA, USA) antibodies, and HRP-conjugated secondary antibodies.
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