Total RNA was extracted from paraffin tissues using FFPE-RNA isolation kit (RTA) and reverse transcribed using cDNA reverse transcription kit (Thermo Fisher) according to manufacturer’s protocols. Quantitative PCR was done using SYBR Green mix (Sigma Aldrich). Reactions were performed in triplicate in a 96-multiwell plate (Roche). Gene expressions were normalized to 18S rRNA, and fold differences were calculated using the comparative CT method: 2-(ΔΔCT), where ΔΔCT refers to (normalized control sample) − (normalized treated sample) as previously described [6 (link)].
96 multiwell plate
Manufactured by Roche
The 96-multiwell plate is a laboratory equipment used for various sample analysis and testing applications. It is a flat plate with an array of small wells arranged in a grid pattern, typically 8 rows by 12 columns, providing a total of 96 individual wells. The wells are designed to hold small volumes of liquids, enabling the simultaneous processing of multiple samples or reagents.
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Lab products found in correlation
2 protocols using 96 multiwell plate
1
Quantitative PCR of FFPE Tissues
2
Quantitative PCR Analysis of RNA
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Total RNA was extracted from para n tissues using FFPE-RNA isolation kit (RTA) and reverse transcribed using cDNA reverse transcription kit (Thermo Fisher) according to manufacturer's protocols. Quantitative PCR was done using SYBR Green mix (Sigma Aldrich). Reactions were performed in triplicate in a 96multiwell plate (Roche). Gene expressions were normalized to 18S rRNA, and fold differences were calculated using the comparative CT method: 2-(ΔΔCT), where ΔΔCT refers to (normalized control sample) -(normalized treated sample) as previously described [6].
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