The largest database of trusted experimental protocols

Bish1475

Manufactured by Sartorius

The BISH1475 is a laboratory equipment product from Sartorius. It is a compact and versatile device designed for various laboratory applications. The core function of the BISH1475 is to provide precise and reliable measurements, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

2 protocols using bish1475

1

Regulation of Fibroblast Activation by UHRF1

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human fibroblasts (MRC-5 cells) and mouse fibroblasts (NIH/3T3) were purchased from ATCC. The PLFs were isolated from the lung of the C57BL/6 mouse. MRC-5 cells were maintained in Minimum Essential Medium (Life Technologies/Gibco), and NIH/3T3 cells and PLFs were maintained in DMEM (Life Technologies/Gibco), which contained 10% fetal bovine serum (BISH1475, Biological Industries) and antibiotics (penicillin and streptomycin, Life Technologies/Gibco). All the cells were cultured at 37°C with 5% CO2. Cells were treated with 5 ng/mL recombinant TGF-β1 (Peprotech) for 48 hours to induce fibroblast activation. To inhibit UHRF1 expression, fibroblasts were treated with 15 μM NSC232003 for 4 hours after TGF-β1 treatment.
UHRF1 siRNA, beclin 1 siRNA, and control siRNA were synthesized by GenePharm. UHRF1 plasmid and beclin 1 plasmid were synthesized by Genery. Cells were transfected using riboFECTCP Reagent (Ribobio) according to the manufacturer’s protocol. The UHRF1 siRNA and beclin 1 siRNA sequences are shown in Supplemental Table 1. The UHRF1 inhibitor NSC232003 was phased from MedChemExpress.
+ Open protocol
+ Expand
2

Investigating Metformin's Modulation of Cell Responses to Silica Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
The lung epithelial cells (A549 and HBE), lung fibroblast (MRC-5), and the human monocytic cell (THP-1) were commercially obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). A549 and THP-1 cells were maintained in RPMI Medium 1640 basic (1640, Life Technologies/Gibco, Grand Island, NY, USA), the HBE cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, 1640, Life Technologies/Gibco, Grand Island, NY, USA), and the MRC-5 cells were maintained in Minimum Essential Medium (MEM, Life Technologies/Gibco, Grand Island, NY, USA). All of the culture media were containing 10% fetal bovine serum (BISH1475, Biological Industries) and antibiotics (penicillin and streptomycin, Life Technologies/Gibco, Gaithersburg, MD). Cells were cultured at 37 °C in a 5% CO2 atmosphere.
PMA (Sigma–Aldrich) was used to treat THP-1 cells into macrophages. For all the experiments analysis, epithelial cells and THP-1 were treated with 200 or 150 μg/ml Silicon dioxide (SiO2) (Sigma-Aldrich, St. Louis, MO, USA) together with various concentrations (2, 5, 10 mM) of metformin (Beyotime Institute of Biotechnology, Shanghai, China); MRC-5 cells were treated with 5 ng/ml TGF-β1 (Sigma-Aldrich) together with metformin.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!