Glutathione peroxidase gpx assay kit

BCA protein assay kit (detection threshold: 50–1000 μg/mL) was Nanjing Aogene Biotech Co., Ltd product (Nanjing, China). Superoxide dismutase (SOD) assay kit, Catalase (CAT) assay kit, Glutathione peroxidase (GPX) assay kit and Malondialdehyde (MDA) assay kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). ATP assay kit (detection threshold: 5 nmol/L-10 μmol/L) and Tissue mitochondria isolation kit were purchased from Beyotime (Jiangsu, China).
DHAV (LQ₂ strain) was obtained from the Shandong Institute of Poultry in China. It was diluted to 10 LD₅₀ (2.5 × 10⁻¹) with sterile saline solution and used in challenge test right after it was ready [24 (link)].

HKC was purchased from Jiangsu Suzhong Pharmaceutical Group Co., Ltd. (Taizhou, Jiangsu, China, batch number: 19101911). One capsule of HKC contains 500 mg extract of A. manihot flowers. Enzyme-linked immunosorbent assay (ELISA) kits for mouse TNF-α and IL-6 were obtained from Biolegend Co. (San Diego, California, USA). The EL-TMB Chromogenic Reagent Kit and 3, 3′, 5, 5′-tetramethylbenzidine (TMB) were purchased from Sangon Biotech Co. (Shanghai, China). Dexamethasone sodium phosphate (DEX) was purchased from Shenyang Guangda Pharmaceutical Co. (Shenyang, Liaoning, China). The glutathione peroxidase (GPx) assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). LPS, TRIzol, Enhanced Cell Counting Kit-8, and dichloro-dihydro-fluorescein diacetate (DCFH-DA) were obtained from Beyotime Biotechnology (Haimen, Jiangsu, China). PrimeScript RT MasterMix was purchased from Takara Biotechnology (Dalian) Co., Ltd. (Dalian, Liaoning, China). HiScript III RT SuperMix for qPCR was obtained from Vazyme Biotech Co., Ltd. (Nanjing, Jiangsu, China). QuantiNova SYBR Green PCR Kit was purchased from Qiagen Co. (Hilden, Germany). All other reagents were of analytical grade.