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2 ml reaction tube

Manufactured by Sarstedt
Sourced in Germany

The 2 mL reaction tubes are versatile laboratory containers used for a variety of sample preparation and storage applications. They provide a convenient and reliable solution for managing small-volume liquid samples.

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4 protocols using 2 ml reaction tube

1

Algae Stress Response Profiling

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Cultures were transferred from standard growth conditions at 12 °C and 100 µmol photons m−2 s−1 to 2, 12, 22 and 32 (±1) °C for one week at 100 and 500 µmol photons m−2 s−1 (n = 8) in a light:dark cycle of 16:8 in a Multi-Cultivator MC 1000-OD (Photon Systems Instruments, Brno, Czech Republic). Additionally, algae were cultured at 2 °C in darkness for one week. Sampling was performed initially (1 h), short-term (16 h) and long-term (1 week), replicates n = 8. An external cooling aggregate was mounted to the MC for the treatments at 2 and 12 °C. Exposures to UV-radiation (UVR) were performed short-term (16 h) at all mentioned temperatures for high and low light (500 and 100 µmol photons m−2 s−1) exposure in a sun simulator (SonSi, iSiTECGmbH, Bremerhaven, Germany). For a solar-like spectrum including UVR (UVA 8.8 Wm−2, UVB 0.61 W m−2), the cultures were illuminated with a 400 W Metallogen lamp (Philips MSR 400 HR, Germany), as described in literature29 (link),30 (link). After exposure, samples from all treatments were collected. Additionally, samples from standard culture conditions and from one day, one week and one month old cultures (n = 3) were taken. Samples were centrifuged in 2 mL reaction tubes (Sarstedt, Nümbrecht, Germany) for 10 s, the liquid phase was discarded. The tubes were initially flash-frozen in liquid nitrogen and stored at −80 °C until RNA extraction.
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2

Polyamine Extraction and Quantification

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Fresh material (200 mg) from selected transgenic HRs grown on solid MS20 medium was transferred to 2-mL reaction tubes (Sarstedt, Nümbrecht, Germany) and powdered by means of metal balls in liquid nitrogen in a Mixer Mill MM 400 (Retsch, Haan, Germany) at a frequency of 30/s for 1 min. The powder was extracted in 1 mL perchloric acid (5%, v/v) containing 2 nmol 1,7-diaminoheptane as an internal standard under freeze-thaw cycles according to Minocha et al. [87 (link)]. Subsequently, the pH was raised to pH 9–10 with 10 M NaOH (approx. 50 μL per 1000 μL extract). Precolumn derivatization and chromatography of polyamines were carried out according to Kaltenegger et al. [88 (link)] using a fluorescence detector (Figure A1).
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3

Gamma Irradiation of Human T Cells

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7.5 × 105 human T cells were transferred in RPMI 1640 medium into a 2 mL reaction tube (Sarstedt, Nümbrecht, Germany). Each reaction tube was treated with a single dose in the range from 2 to 50 Gy as indicated in the respective Figures using the Gammacell® 3000 Elan (Nordion International Inc., Ottawa, ON, Canada).
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4

Sediment Core Sampling and Analysis

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Three replicate sediment cores were sampled with a Kajak gravity corer at each location to give 24 acrylic cores (inner diameter: 7 cm; length: 60 cm) for each bay and a total of 48 cores per sampling occasion as described in Seidel et al. (2022 (link)). Surface plus bottom water temperatures and bottom water oxygen concentrations were measured in situ (Multiline™ sensor, WTW™) during sampling. Additionally, overlying bottom water samples (BW; 50 ml) were taken from the sediment (SED) cores close to the SED surface and transferred into sterile tubes (Thermo Scientific™) for 16S rRNA gene amplicon sequencing (described below). An additional 15 ml of BW was filtered through a 0.7-μm Target2™ GMF Syringe Filter (Thermo Scientific™) into a pre-acid washed polypropylene tube (Thermo Scientific™) for chemical analyses. The BW was decanted from the core, and a 0–1-cm SED slice was collected in a sterile 50-ml polypropylene tube (Thermo Scientific™). The tube contents were well-mixed, and 15 ml was transferred to a pre-acid washed polypropylene tube (Thermo Scientific™) for subsequent pore water analysis. A further 2-ml reaction tube (Sarstedt, Inc.) was filled with SED for organic matter (OM) analysis. All samples were cooled during transport to the laboratory on the same day where they were frozen at either −80 or −20°C.
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