Ptfe coated cell culture insert

Manufactured by Merck Group

The PTFE-coated cell culture insert is a laboratory equipment designed for cell culture applications. The insert is coated with polytetrafluoroethylene (PTFE), a durable and non-stick material, which provides a stable surface for cell growth and adherence. The core function of this product is to facilitate in vitro cell culture studies by providing a controlled environment for cell attachment, proliferation, and differentiation.

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Cell culture inserts (67 citations) PTFE cell culture inserts (2 citations) Culture insert (2 citations)

2 protocols using ptfe coated cell culture insert

Organotypic Brain Slice Culture Analysis

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COCS (350 μm thick) were prepared from 9- to 12-day-old Prnp^0/0, C57BL/6, and tga20 pups according to a previously published protocol (85 (link)). Six to nine COCS were plated per polytetrafluoroethylene (PTFE)–coated cell culture insert (Millipore) in organotypic slice culture medium (85 (link)). Pharmacological treatment was initiated 10 to 14 days after dissection of COCS and was re-added with every medium change [treatment duration: 8 days with three medium changes for Fig. 3 (A to C); Fig. 3D: IgG and 6D11, 14 days; STS, 3 days]. The final concentration of antibodies (6D11, 3F4, and pooled murine IgG) was 67 nM diluted in organotypic slice culture medium. Protein analysis and IHC analysis of COCS were described extensively before (41 (link)).

Linsenmeier L., Mohammadi B., Shafiq M., Frontzek K., Bär J., Shrivastava A.N., Damme M., Song F., Schwarz A., Da Vela S., Massignan T., Jung S., Correia A., Schmitz M., Puig B., Hornemann S., Zerr I., Tatzelt J., Biasini E., Saftig P., Schweizer M., Svergun D., Amin L., Mazzola F., Varani L., Thapa S., Gilch S., Schätzl H., Harris D.A., Triller A., Mikhaylova M., Aguzzi A., Altmeppen H.C, & Glatzel M. (2021). Ligands binding to the prion protein induce its proteolytic release with therapeutic potential in neurodegenerative proteinopathies. Science Advances, 7(48), eabj1826.

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Prion Disease Model in Organotypic Slice Cultures

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350 μm thick COCS were prepared from 9-12 day-old Prnp 0/0 , C57BL/6 and tga20 pups according to a previously published protocol [134] . 6-9 COCS were plated per PTFE-coated cell culture insert (Millipore) in organotypic slice culture medium [134] . Pharmacological treatment was initiated 10-14 days after dissection of COCS and was re-added with every medium change (treatment duration: 8 days with 3 media changes for Fig. 3A-C; Fig. 3D: IgG and 6D11: 14 days, STS: 3 days). Final concentration of antibodies (6D11, 3F4, pooled murine IgG) was 67nM diluted in organotypic slice culture medium. Protein analysis and immunohistochemical analysis of COCS were described extensively before [76] .

Linsenmeier L., Mohammadi B., Shafiq M., Frontzek K., Bär J., Shrivastava A.N., Daμμe M., Schwarz A., Vela S.D., Massignan T., Jung S., Correia Â., Schmitz M., Puig B., Hornemann S., Zerr I., Tatzelt J., Biasini E., Säftig P., Schweizer M., Svergun D.I., Amin L., Mazzola F., Varani L., Thapa S., Gilch S., Schätzl H., Harris D.A., Triller A., Mikhaylova M., Aguzzi A., Altmeppen H.C., & Glatzel M. (2021). Ligands binding to the cellular prion protein induce its protective proteolytic release with therapeutic potential in neurodegenerative proteinopathies.

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