Hplc security guard

Quantitative determination of HPβCD was performed on a reverse-phase, ultra-high-performance liquid chromatography (UHPLC) Ultimate 3000 series system from Dionex Softron GmbH (Germering, Germany) consisting of a LPG-3400SD pump with a built-in degasser, a WPS-3000 autosampler (Dionex, Germering, Germany), a TCC-3100 column compartment (Dionex, Germering, Germany), and a CoronaR ultra RS detector (Dionex, Germering, Germany). During the stationary phase, a Phenomenex Kinetex C18 150 × 4.60 mm 5 µm column with a matching HPLC Security Guard (Phenomenex, Cheshire, UK) were used. The mobile phase consisted of equal volumes of acetonitrile and water; the flow rate was 1.0 mL/min, and the injection volume was 5 µL. Retention time and peak area of all chromatograms were determined using the software ChromeleonR version 7.2 SR4 (ThermoScientific, Waltham, MA, USA).

The HPLC assay was performed using a reverse-phase ultra-high-performance liquid chromatography (UHPLC) Ultimate 3000 series system (Dionex Softron GmbH, Germering, Germany) consisting of a LPG-3400SD pump (Dionex, Germering, Germany) with a built-in degasser, WPS-3000 autosampler (Dionex, Germering, Germany), TCC-3100 column compartment (Dionex, Germering, Germany) and CoronaR ultra RS detector (Dionex, Germering, Germany). During the stationary phase, a Phenomenex Kinetex C18 column (150 × 4.6 mm, 5 µm) with matching HPLC Security Guard (Phenomenex, Cheshire, UK) was used. The mobile phase used a mixture of acetonitrile and water (50:50). The flow rate was 1 mL/min, the column oven temperature was ±25 °C and the detection wave length was set to 254 nm. The retention time for nepafenac under these conditions was 2.3 min.
Peak area and other variables were analysed using the software Chromeleon version 7.2 SR4 (ThermoScientific, Waltham, MA, USA).