For estimation of serum BDNF, 3 ml of venous blood was collected and allowed to clot by leaving it undisturbed at room temperature. The clotted blood was then centrifuged, serum was separated, collected in microcentrifuge tubes and stored in −20°C refrigerator. Serum BDNF was estimated by enzyme-linked immunesorbent assay (ELISA) using human BDNF ELISA kit from Boster Biological Technology Co. Ltd. (Pleasanton, CA, USA). Boster’s human BDNF ELISA kit is based on standard sandwich ELISA technology.
Human bdnf elisa kit
Manufactured by Boster Bio
Sourced in United States
The Human BDNF ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of human Brain-Derived Neurotrophic Factor (BDNF) levels in cell culture supernatants, serum, and plasma samples.
Automatically generated - may contain errors
Lab products found in correlation
Camp assay kit, by R&D Systems (1 mentions)
Brain derived neurotrophic factor (bdnf), by Bioworld Technology (1 mentions)
Protein g agarose, by Merck Group (1 mentions)
Recombinant human bdnf, by Thermo Fisher Scientific (1 mentions)
Pka kinase activity kit, by Abcam (1 mentions)
Acetylcholinesterase activity assay kit, by Merck Group (1 mentions)
Caspase 3 colorimetric activity assay kit, by Merck Group (1 mentions)
4 protocols using human bdnf elisa kit
1
Serum BDNF Quantification Using ELISA
2
Measuring BDNF and NGF Changes
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Blood samples (5 cc) were collected 24 hours before the beginning of the study and 24 hours after the last training session, after 10 hours overnight fasting. Women were evaluated in the follicular stage (first 3 to 5 days of the cycle). BDNF (Human BDNF Elisa kit, Boster Biological Technology Co) and NGF concentration (Human NGF Elisa kit, Boster Biological Technology Co) were measured with ELISA method using specific kits.
3
Molecular mechanisms of BDNF signaling
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Recombinant human BDNF was purchased from PeproTech (Rocky Hill, NJ, USA). FSH (human pituitary) and protein G agarose were from Merck Millipore (Darmstadt, Germany). Hoechst 33342 for DNA staining was obtained from Sigma-Aldrich (Darmstadt, Germany). The following antibodies were used: rabbit polyclonal antibodies against β-actin, FSHR and BDNF (Bioworld Technology, Louis Park, MN, USA); rabbit monoclonal antibodies against CREB and phospho-CREB (Ser133) (Cell signaling Technology, Danvers, MA, USA); rabbit polyclonal antibody against ubiquitin and aromatase, and mouse monoclonal antibody against phosphoserine/threonine/tyrosine (Abcam, Shanghai, CHN). The following kits were employed: Human BDNF ELISA Kit (Boster, Wuhan, CHN); cAMP assay kit (R&D Systems, Inc., Minneapolis, MN, USA); PKA kinase activity kit (Abcam, Shanghai, CHN).
4
Antioxidants and Neuroprotective Factors
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Acetylcholinesterase activity was measured using an acetylcholinesterase activity assay kit (Sigma-Aldrich, St. Louis, USA) based on Ellman’s method. Total antioxidant capacity (TAC) was measured using a complete antioxidant capacity test kit (Bio Diagnostic Co. in Cairo, Egypt) based on the method described by Koracevic et al. [17 (link)]. Brain-derived neurotrophic factor levels were measured using a Human BDNF ELISA kit from Boster Biological Technology (Encyclopedia Cir., Fermont, CA), according to the manufacturer’s instructions. Brain BDNF levels were expressed as pg/g of tissue [18 (link)]. Activity levels of the endogenous antioxidant enzyme superoxide dismutase (SOD) were measured using SOD assay kit (Caymanv Chemicals Company, Ann Arbor, MI, USA) following the manufacturer’s instructions. Superoxide dismutase activity was expressed as U/mg of protein. Caspase 3 activity was measured using the Caspase-3 Colorimetric Activity Assay Kit (Chemicon, Temecula, USA) [19 (link)].
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