Abi 5500 qtrap

The total drug is measured in our study including released and unreleased drug, as well as protein bound drug in plasma to illustrate the questions below, unlike other studies provided the detail analysis of three different types of drugs [51 (link),52 ], The LC-MS/MS analysis of paclitaxel was performed using docetaxel as internal standard (IS). LC-MS/MS analysis of doxorubicin was performed using daunarubicin as internal standard (IS). The analytical assay was validated according FDA guidance for linearity (2–5000 ng/mL), matrix effect, recovery, low detection limit, Quality control (QC) in different biological matrix, including plasma, blood, tumor, and each different organ homogenates. LC-MS/MS was performed using ABI-5500 Qtrap (Sciex, Ontario, Canada) mass spectrometer with electrospray ionization source was interfaced with Shimadzu high performance liquid chromatography (HPLC) system with Xbridge C18 column (50 × 2.1 mm ID, 3.5 μm). The mass spectrometer was operated in a positive mode with multiple reaction monitoring (MRM) for analysis. The MRM transitions were monitored at m/z 854.4 to 286.1 for paclitaxel and m/z 808.0 to 226.0 for internal standard. The MRM transitions were monitored at m/z 544.1 to 397.2 for doxorubicin and m/z 528.5 to 321 for internal standard.