The siRNA library consisting of 87 human DUBs (#4392425) was purchased from Applied Biosystems (Ambion Silencer siRNA Custom Library). Different siRNAs were transfected with lipofectamine 2000 (Invitrogen, Carlsbad, CA) into HeLa cells in 24-well plates for two days. Cell lysates were extracted and the protein levels of endogenous KLF5 were measured by Western blotting (WB).
Ambion silencer sirna custom library
Manufactured by Thermo Fisher Scientific
The Ambion Silencer siRNA Custom Library is a collection of small interfering RNA (siRNA) molecules designed for gene silencing experiments. The library allows researchers to target specific genes of interest and study their functions by selectively reducing their expression in cells. The siRNA sequences are custom-designed to provide efficient and specific gene knockdown.
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Lab products found in correlation
2 protocols using ambion silencer sirna custom library
1
Screening of Human Deubiquitinases for KLF5 Regulation
2
Screening DUB siRNA Library for KLF5 Regulation
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The siRNA library consisting of 87 human DUBs was purchased from Applied Biosystems (Ambion Silencer siRNA Custom Library, catalogue number 4392425).
Different siRNAs were transfected with LF2000 (Invitrogen, #11668-019) into HeLa cells in 24-well plates for 2 days. Cell lysates were extracted and the protein levels of endogenous KLF5 were measured by WB. We identified 27 candidates in the first round of screening. The candidate genes were further validated in MCF10A and HeLa cells.
Different siRNAs were transfected with LF2000 (Invitrogen, #11668-019) into HeLa cells in 24-well plates for 2 days. Cell lysates were extracted and the protein levels of endogenous KLF5 were measured by WB. We identified 27 candidates in the first round of screening. The candidate genes were further validated in MCF10A and HeLa cells.
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