Slit gal4

Manufactured by Bloomington Drosophila Stock Center

The Slit-Gal4 is a genetic tool used in Drosophila research. It drives the expression of the Gal4 transcriptional activator in a specific pattern during development, allowing for targeted manipulation of gene expression in the fruit fly.

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Lab products found in correlation

Ok371 gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions) Repo gal4, by Bloomington Drosophila Stock Center (1 mentions) Tubulin gal4, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using slit gal4

Fly Strains for Neuronal Connectivity Research

Cited 2 times

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The following fly strains were used;
OK371-Gal4,UAS-mCD8GFP, UAS-dicer; OK371-Gal4,UAS-mCD8GFP, Repo-Gal4, Slit-Gal4, VGN9281-Gal4,UAS-mCD8GFP (Brierley et al., 2009 (link)), VGN6341-Gal4,UAS-mCD8GFP, R60B11-Gal4 (39238; Bloomington Drosophila Stock Center). RNAi lines and mutants used in these experiments have been described previously. UAS Sema1a RNAi (VDRC, TRiP, Gift from Alex Kolodkin (Sweeney et al., 2007 (link)), UAS PlexA RNAi (VDRC; Gift from Liqun Luo (Sweeney et al., 2007 (link); Pecot et al., 2013 (link)), UAS Sema2a RNAi (VDRC (Sweeney et al., 2011 (link)), UAS PlexB RNAi (VDRC, TRiP), CA07125 (Fly trap), Sema1a P¹ and Sema1a P² (Yu et al., 1998 (link)), plexADf(4)C3 (PlexA⁰⁹)(Winberg et al., 1998 (link)), plexB^KG00878 (Ayoob et al., 2006 (link); Bellen et al., 2004 (link)).

Syed D.S., Gowda S.B., Reddy O.V., Reichert H, & VijayRaghavan K. (2016). Glial and neuronal Semaphorin signaling instruct the development of a functional myotopic map for Drosophila walking. eLife, 5, e11572.

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Drosophila Genetic Manipulation Protocols

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Flies were maintained on standard cornmeal-agar medium at 29°C unless otherwise indicated. dTet-GFP flies were kindly provided by Ruth Steward. Tubulin-Gal4 (#5138), Slit-Gal4 (#9580), and Sim-Gal4 (#9150) fly stocks were obtained from Bloomington Drosophila Stock Center. dTet-RNAi (#102273 and #36187) and mCherry-RNAi (#35785) flies were obtained from Vienna Drosophila RNAi Center.

Ismail J.N., Badini S., Frey F., Abou-Kheir W, & Shirinian M. (2019). Drosophila Tet Is Expressed in Midline Glia and Is Required for Proper Axonal Development. Frontiers in Cellular Neuroscience, 13, 252.

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