Proteins of cell lysates or tissue homogenates were separated by SDS–PAGE according to their molecular weight using Tris/glycine as electrophoresis buffer and were transferred onto polyvinylidene fluoride membranes (Carl Roth GmbH, Karlsruhe, Germany) using CAPS buffer. After blocking, membranes were hybridized with respective primary antibodies. Membranes were washed, incubated with respective secondary horseradish-peroxidase (HRP)-conjugated antibody and detected using ECL2 Western blotting substrate (Thermo Scientific, Waltham, MA). Antibodies used were rabbit anti-HSL, rabbit anti-phospho-HSL (S660), and rabbit anti-GAPDH (all from Cell Signaling, Danvers, MA, USA), rabbit anti-CGI-58 (Abnova, Heidelberg), mouse anti-β-Actin (Santa Cruz, Santa Cruz, CA, USA), rabbit anti-alpha smooth muscle actin (α-SMA, Pierce, Thermo Scientific), HRP-linked sheep-anti mouse antibody, (GE Healthcare Amersham, Buckinghamshire, UK), and HRP-linked rat-anti rabbit antibody (Dako, Glostrup, Denmark).
Rabbit anti phospho hsl s660
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-phospho-HSL (S660) is a primary antibody that detects the phosphorylated form of hormone-sensitive lipase (HSL) at serine 660. It is used to identify and quantify the phosphorylation of HSL at this specific site.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride membrane, by Carl Roth (2 mentions)
Rabbit anti cgi 58, by Abnova (2 mentions)
Hrp linked sheep anti mouse antibody, by GE Healthcare (2 mentions)
Hrp linked rat anti rabbit antibody, by Agilent Technologies (2 mentions)
Ecl2 western blotting substrate, by Thermo Fisher Scientific (2 mentions)
Rabbit anti hsl, by Cell Signaling Technology (2 mentions)
Mouse anti β actin, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (2 mentions)
2 protocols using rabbit anti phospho hsl s660
1
Western Blot Analysis of Lipid Metabolism Proteins
2
Western Blot Analysis of Lipid Metabolism Proteins
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Proteins of cell lysates or tissue homogenates were separated by SDS–PAGE according to their molecular weight using Tris/glycine as electrophoresis buffer and were transferred onto polyvinylidene fluoride membranes (Carl Roth GmbH, Karlsruhe, Germany) using CAPS buffer. After blocking, membranes were hybridized with respective primary antibodies. Membranes were washed, incubated with respective secondary horseradish-peroxidase (HRP)-conjugated antibody and detected using ECL2 Western blotting substrate (Thermo Scientific, Waltham, MA). Antibodies used were rabbit anti-HSL, rabbit anti-phospho-HSL (S660), and rabbit anti-GAPDH (all from Cell Signaling, Danvers, MA, USA), rabbit anti-CGI-58 (Abnova, Heidelberg), mouse anti-β-Actin (Santa Cruz, Santa Cruz, CA, USA), rabbit anti-alpha smooth muscle actin (α-SMA, Pierce, Thermo Scientific), HRP-linked sheep-anti mouse antibody (GE Healthcare Amersham, Buckinghamshire, UK), and HRP-linked rat-anti rabbit antibody (Dako, Glostrup, Denmark).
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