The protocols used to process root tip samples and to perform multicolour FISH followed those described by Komuro et al. [40 (link)] and Zeng et al. [39 (link)]. The oligonucleotide probes Oligo-pSc119.2 and Oligo-pTa535 were synthesized by TSINGKE Biological Technology Company (Beijing, China) and were 5′ end labelled with either TAMRA or 6-FAM. Genomic in situ hybridization (GISH) experiments were performed as described by Hao et al. [41 ]. To distinguish between the chromosome complements of each of the three wheat subgenomes, total genomic DNA extracted from the A genome donor Triticum urartu and the D genome donor Ae. tauschii was labelled with digoxigenin-11-dUTP and biotin-16-dUTP (Roche Diagnostics GmbH, Mannheim, Germany), respectively. Unlabelled genomic DNA from Aegilops speltoides (putative B genome donor) was used for blocking.
Oligo pta535
Manufactured by Tsingke
Sourced in China
The Oligo-pTa535 is a laboratory equipment used for the synthesis of oligonucleotides. It is a specialized instrument designed to automate the process of synthesizing short DNA or RNA sequences. The core function of the Oligo-pTa535 is to efficiently produce custom-designed oligonucleotides for various applications in molecular biology, genetics, and biotechnology research.
Automatically generated - may contain errors
2 protocols using oligo pta535
1
Multicolor FISH Protocol for Wheat
2
Chromosome Identification Using FISH Probes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seed germination and root tip collection and processing were as described by Zhao et al. (2018) . Slides were prepared as described by Komuro et al. (2013) . Two oligonucleotide (oligo) probes with 6-FAM or Tamra label at the 5'-end, oligo-pSc119.2 and oligo-pTa535 (Tang et al., 2014) , were synthesized by the TsingKe Biological Technology Company (Chengdu, China) and used to identify chromosome constitution. oligo-pSc119.2 was used to detect B-and R-genome chromosomes, and oligo-pTa535 was used to detect A-and D-genome chromosomes. The FISH procedure was conducted according to methods described by Zhao et al. (2018) . Photomicrographs of chromosome observations were created and documented using an Olympus BX-63 microscope (Tokyo, Japan) coupled to a Photometric SenSys Olympus DP80 CCD camera. Karyotype analysis was carried out on 5 accessions selected according to read coverage of GBS results.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!