ISE6 tick cells (approximately 5 × 105 cells/well) were inoculated with A. phagocytophilum NY18 then sampled at 7 days post-infection (dpi, % infected cells > 70%). Uninfected cells were included as controls. Harvested cells were used to determine the concentration of glycerol 3-phosphate (G-3P) and PEP using the glycerol 3-phosphate colorimetric assay Kit (Sigma Cat. No. MAK207) or the PEP colorimetric/fluorometric assay kit (Sigma Cat. No. MAK102) respectively, following manufacturer's protocols. G-3P and PEP levels (ng/μl) were compared between untreated and treated cells by Student's t-test with unequal variance (P < 0.05; N = 4 biological replicates).
Pep colorimetric fluorometric assay kit
Manufactured by Merck Group
The PEP Colorimetric/Fluorometric Assay Kit is a laboratory tool used to measure the activity of the enzyme Phosphoenolpyruvate (PEP) carboxylase. The kit utilizes either a colorimetric or fluorometric method to quantify PEP levels in samples.
Automatically generated - may contain errors
Lab products found in correlation
6 phosphogluconate assay kit, by Abcam (2 mentions)
Atp colorimetric fluorometric assay kit, by Merck Group (2 mentions)
Pyruvate assay kit, by Merck Group (2 mentions)
Adp colorimetric fluorometric assay kit, by Merck Group (2 mentions)
Glycerol 3 phosphate colorimetric assay kit, by Merck Group (1 mentions)
Infinite 200 pro multi well plate reader, by Tecan (1 mentions)
4 protocols using pep colorimetric fluorometric assay kit
1
Metabolic Changes in Tick Cells Infected with Anaplasma
2
Quantification of Metabolites in Kidney AKI
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The amount of pyruvate was measured using Pyruvate Assay Kit (Sigma). Kidneys harvested from Akr1a1+/+, Akr1a1−/−, Pkm2+/+ or Pkm2−/− mice (sham operation or AKI) were mechanically homogenized in Pyruvate Assay Buffer (1 mg/5 μl buffer). After extracts were clarified by centrifugation (20,000g, 4 °C, 20 min, ×2), supernatant was used for assay. GHB in the serum of Akr1a1+/+ and Akr1a1−/− mice was measured following the GHB enzymatic assay kit from BUHLMANN. For measuring PEP, 6PG, ATP, ADP and serine in HEK cells, 1 × 106 cells were lysed in corresponding buffer. The amount of PEP, 6PG, ATP, ADP and serine were respectively measured using PEP Colorimetric/Fluorometric Assay Kit (Sigma), 6 Phosphogluconate Assay kit (abcam), ATP Colorimetric/Fluorometric Assay Kit (Sigma), ADP Colorimetric/Fluorometric Assay Kit (Sigma) and DL-Serine Assay kit (Fluorometric) (Biovision).
3
Quantifying PEP in S. coelicolor
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The PEP Colorimetric/Fluorometric Assay Kit from Sigma-Aldrich was used to determine the PEP concentration in S. coelicolor according to the manufacturer’s protocol. 2–4 mg of mycelium harvested from solid medium were used for the enzymatic reaction. Signals were quantified by fluorescence using a Tecan infinite 200 pro multiwell plate reader.
4
Quantification of Metabolites in Kidney AKI
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