The following constructs were obtained from Addgene: pcDNA3/Myc-DNMT1 (plasmid 36939, from A. Riggs), pRK5-HA-Ubiquitin-WT (plasmid 17608, from T. Dawson), pCDNA3 Flag MKK3b(Glu) (plasmid 50449, from R. Davis), and pcDNA3 Flag HA (plasmid 10792, from W. Sellers). We obtained pCMV6-AC-GFP-NOS2 (NOS2-GFP) from OriGene (RG211819), and pcDNA3.1-HA-KAT5 (isoform 3) and pcDNA3.1-HA-KAT5 (isoform 3; T191A) were synthesized by GenScript. Cells (0.25 × 106 in 3 mL of medium) were seeded into 6-well plates and cultured overnight. Plasmids were diluted in Opti-MEM medium (125 µL/well) and supplemented with P3000 (5 µL/well) and added to Lipofectamine 3000 (5 µL/well) diluted in Opti-MEM medium (125 µL/well). DNA complexes were incubated for 15 min at room temperature and added dropwise to cells. Mock-transfected cells were treated with Lipofectamine and P3000 diluted in Opti-MEM as described. Cells were then incubated for the times indicated before analysis.
Switzer C.H., Cho H.J., Eykyn T.R., Lavender P, & Eaton P. (2022). NOS2 and S-nitrosothiol signaling induces DNA hypomethylation and LINE-1 retrotransposon expression. Proceedings of the National Academy of Sciences of the United States of America, 119(21), e2200022119.
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