Ab176184

Rabbit polyclonal antibodies against GRP78 (ab188878), CHOP (ab179823), and NURR1 (ab176184); the mouse monoclonal antibody against MAP2 (ab11268); and the rabbit monoclonal antibody targeting Ki-67 (ab16667) were purchased from Abcam (United States). Rabbit polyclonal antibodies against p-eIF2α (AF3087) and ATF6 (DF6009) were purchased from Affinity (China). The Alexa Fluor 488 donkey anti-mouse IgG (H + L) (1975519) and the Alexa Fluor 594 donkey anti-rabbit IgG (H + L) (1827674) secondary antibodies were purchased from Invitrogen (United States). The immunohistochemistry kit (SP9001) was purchased from the Zhongshan Goldenbridge Biotech, China, and the morphine hydrochloride for injection was produced in the First Pharmaceutical Factory of Shenyang, China.

Proteins were extracted from cells and brain tissues and treated with RIPA lysis and extraction buffer (KeyGen Biotechnology, Nanjing, China), and then, the concentrations of these samples were measured by bicinchoninic acid (BCA) assay. The specific steps of Western blotting were the same as those described in our previous studies [17 (link)]. The antibodies were anti-CD63 (ab217345, Abcam), anti-TSG101 (ab125011, Abcam), anti-Calnexin (ab10286, Abcam), anti-Nr4a2 (ab176184, Abcam), anti-NF-κB p65 (ab16502, Abcam) and anti-GAPDH (ab9485, Abcam). Cell culture experiments were performed in triplicates.