Intellipath flx automated stainer

Manufactured by Biocare Medical

Sourced in United States

The IntelliPATH FLX Automated Stainer is a laboratory equipment designed for the automated staining of histological and cytological samples. It performs various staining protocols on tissue sections or cell preparations to enable visualization and analysis under a microscope.

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Lab products found in correlation

Decloaking chamber, by Biocare Medical (2 mentions) Mach 2 hrp polymer, by Biocare Medical (1 mentions) 3 3 diaminobenzidine dab chromogen, by Agilent Technologies (1 mentions) 3 diaminobenzidine dab chromogen, by Agilent Technologies (1 mentions) Superfrost slide, by Thermo Fisher Scientific (1 mentions) Prism 6, by GraphPad (1 mentions)

Close spelling variants of this product

IntelliPATH FLX Intellipath

3 protocols using intellipath flx automated stainer

Immunohistochemistry Staining Protocol

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Antibodies used for IF and IHC are listed in supplementary material, Table S1. Paraffin sections cut at 5 μm were mounted on Fisherbrand Superfrost slides (Thermo Fisher Scientific, Pittsburgh, PA, USA), incubated for 60 min at 60 °C, and then deparaffinized and rehydrated. Epitope retrieval was performed in a Decloaking Chamber (Biocare Medical, Pacheco, CA, USA) under pressure for 5 min using citrate buffer, pH 6.0, followed by a 10‐min cooling down period. Endogenous peroxidase was blocked using 3% H₂O₂ for 10 min, followed by incubation with primary antibody for 40 min followed by Mach 2 HRP‐Polymer (BioCare, Concord, CA, USA) for 30 min, and 3,3'‐diaminobenzidine (DAB)+chromogen (Dako, Carpinteria, CA, USA) for 5 min. Immunohistochemical staining was performed using an IntelliPATH FLX Automated Stainer (Biocare Medical, Pacheco, CA, USA) at room temperature. Light hematoxylin counterstaining was performed, after which the slides were dehydrated, cleared, and mounted using a permanent mounting medium.

Hong Y., Limback D., Elsarraj H.S., Harper H., Haines H., Hansford H., Ricci M., Kaufman C., Wedlock E., Xu M., Zhang J., May L., Cusick T., Inciardi M., Redick M., Gatewood J., Winblad O., Aripoli A., Huppe A., Balanoff C., Wagner J.L., Amin A.L., Larson K.E., Ricci L., Tawfik O., Razek H., Meierotto R.O., Madan R., Godwin A.K., Thompson J., Hilsenbeck S.G., Futreal A., Thompson A., Hwang E.S., Fan F, & Behbod F. (2021). Mouse‐INtraDuctal (MIND): an in vivo model for studying the underlying mechanisms of DCIS malignancy. The Journal of Pathology, 256(2), 186-201.

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Immunohistochemical Staining Protocol

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Antibodies used for IF and IHC are listed in supplementary material, Table S1. Paraffin sections cut at 5 μm were mounted on Fisherbrand Superfrost slides (Pittsburgh, PA, USA), incubated for 60 min at 60 °C and then deparaffinized and rehydrated. Epitope retrieval was performed in a Decloaking Chamber (Biocare Medical, Pacheco, CA, USA) under pressure for 5 min using citrate buffer pH 6.0, followed by a 10-min cooling down period. Endogenous peroxidase was blocked using 3% H₂O₂ for 10 min, followed by incubation with primary antibody for 40 min followed by Mach 2 HRP Polymer BioCare (Concord, CA, USA) for 30 min, and 3-diaminobenzidine (DAB)+ chromogen (Dako, Carpinteria, CA, USA) for 5 min. Immunohistochemical staining was performed using an IntelliPATH FLX Automated Stainer (Biocare Medical, Pacheco, CA, USA) at room temperature (RT). Light hematoxylin counterstaining was performed, after which the slides were dehydrated, cleared, and mounted using a permanent mounting medium.

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Immunohistochemical Analysis of JNA Samples

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IHC was performed on tissue obtained from patient paraffin samples. Twenty-eight JNA, 8 normal adjacent, and 2 additional samples from tumor-free patients were included (age JNA: median = 16.5 years, interquartile range = 5.75 years; normal: median = 15.5 years, interquartile range = 6 years).
Primary antibodies fibroblast growth factor receptor 2 (FGFR2; E10570) and vascular endothelial growth factor (VEGF; E2610) (Spring Bioscience, Pleasanton, California) were used. Immunohistochemical staining was performed using the IntelliPATH FLX Automated Stainer (Biocare Medical, Pachecho, California). Staining intensity (0 indicates no staining, 1+ weak staining, 2+ moderate, and 3+ strong staining) was determined by a board-certified pathologist (O.T.). Significant differences were determined by nonparametric Mann-Whitney U tests (GraphPad Prism 6, version 6.03; GraphPad Software, La Jolla, California).

Jones J.W., Usman S., New J., Holcomb A., Gunewardena S., Tawfik O., Hoover L., Bruegger D, & Thomas S.M. (2018). Differential Gene Expression and Pathway Analysis in Juvenile Nasopharyngeal Angiofibroma Using RNA Sequencing. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 159(3), 572-575.

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