The CDCA3 antibody (HPA026587), monoclonal FLAG M2 antibody (F1804) and α-Tubulin antibody (T9026) were purchased from Sigma Aldrich. Antibodies against the HA tag (#3724) and phospho-CK2 substrate motif (#8738) were purchased from Cell Signaling Technology (Genesearch, Australia). The Cdh1 (ab77885) and γH2AX (ab26350) antibodies were from Abcam. The FANCI antibody (A301-254A) was from Bethyl Laboratories, Inc. 4′-6-diamidino-2-phenylindole (DAPI) was from Life Technologies and Complete EDTA-free protease inhibitor mixture was from Roche Applied Sciences. Donkey anti-rabbit and anti-mouse Alexa Fluor 488 antibodies were purchased from Life Technologies. CX-4945 was purchased from Selleck Chemicals Llc and proTAME was purchased from BostonBiochem. All other reagents were from Sigma-Aldrich except where noted. Mammalian expression construct for HA-Cdh1 was a gift from Marc Kirschner (Addgene plasmid #1159651 (link)) and the CDCA3-FLAG expression construct was generated by subcloning the full-length CDCA3 sequence in pcDNA3.1+ using the BamHI and EcoRI restriction enzyme sites. N-terminal consensus kozak sequence and C-terminal triple FLAG sequence were introduced using PCR.
Donkey anti rabbit and anti mouse alexa fluor 488 antibodies
Manufactured by Thermo Fisher Scientific
Thermo Fisher Scientific's Donkey anti-rabbit and anti-mouse Alexa Fluor 488 antibodies are fluorescently labeled secondary antibodies. They are designed to detect and bind to primary antibodies raised in rabbit or mouse, and the Alexa Fluor 488 dye allows for fluorescent visualization of the targeted proteins or molecules.
Automatically generated - may contain errors
Lab products found in correlation
Ab26350, by Abcam (1 mentions)
Cx 4945, by Selleck Chemicals (1 mentions)
Protame, by R&D Systems (1 mentions)
α tubulin antibody t9026, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Complete edta free protease inhibitor mixture, by Roche (1 mentions)
Anti p53 clone d0 7, by Merck Group (1 mentions)
2 protocols using donkey anti rabbit and anti mouse alexa fluor 488 antibodies
1
Cdh1 and CDCA3 Regulation Assay
2
Antibody Procurement for Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-CDCA3 antibody (HPA026587) and anti-p53 clone D07 were purchased from SigmaAldrich (Castle Hill, NSW, Australia). Anti-Actin and anti-p21 antibodies were purchased from Cell Signaling Technology (Genesearch, Gold Coast, Australia). 4 0 -6-Diamidino-2-phenylindole was purchased from Life Technologies (Mulgrave, Australia) and complete ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor mixture was obtained from Roche Applied Sciences (Castle Hill, NSW, Australia). Donkey anti-rabbit and anti-mouse Alexa Fluor 488 antibodies were purchased from Life Technologies. All other reagents were from SigmaAldrich except where noted.
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