Mouse anti k7 rck 105

Primary antibodies used for Western blotting and immunofluorescence staining were mouse anti-K7 (RCK-105; Progen, Heidelberg, Germany), rat anti-K8 and rat anti-K19 (Troma I and Troma III, respectively; Developmental Studies Hybridoma Bank, Iowa, IA, USA), rabbit anti-K8 (273) and rabbit anti-K18 (275; kind gifts from J.E. Eriksson), rabbit anti-K20 (It-Ks 20.10; Epitomics, Burlingame, CA, USA), rat anti-Hsc70 (Enzo Life sciences; Farmingdale, NY, USA), mouse anti-K8 pS74 (LJ4; kind gift from M.B. Omary), rabbit anti-Ki67 (Abcam, Cambridge, MA, USA), rat anti-HSF2 (Abcam) and rabbit anti-IκB-α (Santa Cruz Biotechnology; Dallas, TX, USA). Secondary antibodies used for Western blotting were HRP-conjugated anti-mouse (GE healthcare, Little Chalfont, UK), anti-rat (GE healthcare and Cell Signaling Technology, Danvers, MA, USA) and anti-rabbit (Cell Signaling Technology) IgG antibodies. Secondary antibodies used for immunofluorescence staining were Alexa 488/Alexa 546 anti-mouse, Alexa 488 anti-rat and Alexa 488 anti-rabbit antibodies (Invitrogen, Carlsbad, CA, USA). Nuclei were stained with DRAQ5 (Cell Signaling Technology).