RAW264.7 cell, ARPE-19 cell, MOVAS cell, SVEC, and fetal bovine serum (FBS), phosphate buffered saline (PBS), Dulbecco's modified eagle medium (DMEM) were all purchased from ATCC (Manassas, VA). L-929 cell and mouse primary tenocyte (kindly provided by Dr. Rupak Rajachar, Houghton, MI). Silanol-terminated PDMS, (3-aminopropyl) trimethoxylsilane, dibutyltin dilaurate were purchased from Gelest Inc. (Morrisville, PA). Room temperature vulcanized PDMS (RTV-3140) and silicone elastomer base and curing agent (Sylgard® 184) were acquired from Dow Corning Co. (Midland, MI). Interferon-γ mouse was obtained from BD Biosciences (San Diego, CA), lipopolysaccharine, L-arginine, N-acetyl-D, L-penicillamine, acetyl anhydrate, calcein-AM, and 4-amino-5-methylamino-2′,7′-difluorescein (DAF-FM), and cyclam were obtained from Sigma-Aldrich (St. Louis, MO). Tertbutyl nitrite was purchased from Acros Organics, (Pittsburgh, PA). Toluene was purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Nω-hydroxy-nor-Arginine (nor-NOHA), L-NG-Nitroarginine methyl ester, and nitrate/nitrite colorimetric assay kit were obtained from Cayman Chemical (Ann Arbor, MI). Gelatin was obtained from Bio-rad (Hercules, CA). Pyridine was purchased from EMD Chemical Inc. (Darmstadt, Germany). Ethidium bromide was obtained from Invitrogen (Grand Island, NY).
4 amino 5 methylamino 2 7 difluorescein daf fm
Manufactured by Merck Group
Sourced in United States
4-amino-5-methylamino-2′,7′-difluorescein (DAF-FM) is a fluorescent dye used for the detection and quantification of nitric oxide (NO) in biological samples. It reacts with NO to form a highly fluorescent benzotriazole derivative, allowing for the sensitive and specific measurement of NO levels.
Automatically generated - may contain errors
Lab products found in correlation
Nitrate nitrite colorimetric assay kit, by Cayman Chemical (1 mentions)
Raw264.7 cell, by American Type Culture Collection (1 mentions)
Arpe 19 cell, by American Type Culture Collection (1 mentions)
Ethidium bromide, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
Calcein am, by Merck Group (1 mentions)
L arginine, by Merck Group (1 mentions)
Dulbecco s modified eagle medium, by American Type Culture Collection (1 mentions)
Tert butyl nitrite, by Thermo Fisher Scientific (1 mentions)
Gelatin, by Bio-Rad (1 mentions)
Cyclam, by Merck Group (1 mentions)
L penicillamine, by Merck Group (1 mentions)
Toluene, by Mallinckrodt (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Nih 3t3, by Leibniz Institute DSMZ (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
2 protocols using 4 amino 5 methylamino 2 7 difluorescein daf fm
1
Cellular and Material Characterization
2
Dendrimer-Mediated Cytotoxicity Evaluation
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PAMAM-NH2 dendrimer generation 0 (20 wt.% solution in methanol) was acquired from Sigma-Aldrich (Germany, manufactured by Dendritech, Midland, MI, USA). Chlorambucil was acquired from Alfa Aesar (Karlsruhe, Germany). HT-29 cells were provided by Professor B. Seliger (Immunology Department, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany). PC-3 and NIH3T3 cell lines were purchased from German Collection of Microorganisms and Cell Cultures (Leibniz-DSMZ, Braunschweig, Germany). Fetal calf serum (FCS), RPMI-1640, phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), 3-methyl adenine (3-MA), carboxyfluorescein diacetate succinimidyl ester (CFSE), 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), crystal violet (CV), 4′,6-diamidino-2-phenylindole (DAPI), ethylenediamine tetraacetic acid (EDTA), propidium iodide (PI), and 4-amino-5-methylamino-2′,7′-difluorescein (DAF-FM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Annexin V-FITC (AnnV) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Acridine orange (AO) was from Labo-Moderna (Paris, France). All other chemicals and reagents purchased from commercial source were obtained from Sigma-Aldrich (Taufkirchen, Germany) or Alfa Aesar (Karlsruhe, Germany), and were used without further purification.
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