Aptima ct single assay

Manufactured by Hologic

The Aptima CT single assay is a laboratory diagnostic test designed to detect the presence of Chlamydia trachomatis (CT) in clinical samples. The assay utilizes a proprietary target capture and amplification technology to provide accurate and reliable results for the identification of CT infections.

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Lab products found in correlation

Aptima combo 2, by Hologic (1 mentions) Aptima, by Hologic (1 mentions)

2 protocols using aptima ct single assay

Detection of MG, CT, and NG using TMA

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The MG-transcription mediated amplification (TMA) assay (Hologic Inc, San Diego, USA) was used for the detection of MG and the Aptima Combo 2 (AC2) TMA was used for the detection of CT and NG on the Panther system according to the manufacturer's instructions. Samples with equivocal results were retested by using the Aptima CT single assay (Hologic) for CT, and an NG qPCR targeting the opa genes for NG [20 (link)].

Braam J.F., Hetem D.J., Vergunst C.E., Kuizenga Wessel S., van Rooijen M.S., Nijhuis R.H., Schim van der Loeff M.F., van Dam A.P, & Bruisten S.M. (2020). Evaluating the prevalence and risk factors for macrolide resistance in Mycoplasma genitalium using a newly developed qPCR assay. PLoS ONE, 15(10), e0240836.

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Sexually Transmitted Infection Screening Protocol

Cited 1 time

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First-void urine was collected and routinely tested for NG and CT (Aptima Combo 2; Hologic Inc, San Diego, CA). Samples with equivocal results were retested using the Aptima CT single assay (Hologic Inc) and for NG with a quantitative polymerase chain reaction targeting opa genes.^{17 (link)} Urine samples were tested for MG using transcription-mediated amplification (TMA) assays (Aptima, Hologic Inc). From all samples that tested positive for MG in the MG-TMA assay, DNA was extracted with isopropanol precipitation and subsequently tested for macrolide resistance using the MG-MRAM quantitative polymerase chain reaction to detect wild-type (MG-WT) or any mutations (MRAM) in the 23S rRNA gene at nucleotide positions 2058 and 2059 (Escherichia coli numbering).^{18 (link)}Mycoplasma genitalium and MRAM testing were done for study purposes only, and results were disclosed neither to the men nor to the healthcare professionals. A subset of samples was tested for Trichomonas vaginalis (TV) using TMA assays (Aptima, Hologic Inc).

Braam J.F., van Dam A.P., Bruisten S.M., van Rooijen M.S., de Vries H.J., Schim van der Loeff M.F, & Vergunst C.E. (2021). Macrolide-Resistant Mycoplasma genitalium Impairs Clinical Improvement of Male Urethritis After Empirical Treatment. Sexually Transmitted Diseases, 49(5), 360-367.

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