Fresh cut 6 μm thick frozen tissue sections were fixed in 1% paraformaldehyde for 20 min., washed in TBS and blocked 20 min. with 1% hydrogen peroxide, and then washed in TBS followed by blocking with 2.5% normal horse serum (Vector labs). Sections were stained with mouse anti p24 (DAKO, Carpintera, CA) for 1 hour, washed and protein detected using ImmPRESS peroxidase kit for mouse (Vector Labs) and Nova Red Substrate (Vector Labs) as described above. Sections were blocked a second time with 2.5% horse serum and stained with mouse anti human CD20 (DAKO) and detected using ImmPRESS peroxidase kit for mouse and Vector SG (Vector Labs) as the substrate. Slides were briefly counterstained with 50% hematoxylin and coverslipped. The percent of p24 staining in the follicle was determined by quantitative image analysis using Qwin Pro (Leica v 3.4.0) on a Leica DMR brightfield microscope by determing the area of the follicle positive for p24 and dividing by the total area of the follicle.
Dmr bright field microscope
Manufactured by Leica
The DMR bright-field microscope is a laboratory equipment designed for high-quality imaging and observation of specimens. It provides clear and detailed views of samples using bright-field illumination technology. The core function of the DMR microscope is to enable users to examine and analyze various materials and specimens with precision and clarity.
Automatically generated - may contain errors
Lab products found in correlation
No dfc480, by Leica (2 mentions)
Normal horse serum, by Vector Laboratories (1 mentions)
Horse serum, by Agilent Technologies (1 mentions)
Qwin pro, by Leica (1 mentions)
Mouse anti human cd20, by Agilent Technologies (1 mentions)
Immpress peroxidase kit for mouse, by Vector Laboratories (1 mentions)
Novared substrate, by Vector Laboratories (1 mentions)
3 protocols using dmr bright field microscope
1
Quantitative Analysis of p24 and CD20 Immunohistochemistry
2
Histological Analysis of Frozen Muscle
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OCT-embedded frozen muscle was cross-sectioned into 10 µm thick sections, stained with Hematoxylin & Eosin (H&E) or picrosirius red as previously described (Hammers et al., 2020 (link)), and visualized with a Leica DMR bright-field microscope equipped with a digital camera (Leica No. DFC480). Image analysis was performed using FIJI software.
3
Muscle Histology with OCT Sections
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OCT-embedded frozen muscle was cross-sectioned into 10 µm thick sections, stained with Hematoxylin & Eosin (H&E) or picrosirius red (PSR) as previously described (Hammers et al., 2020) , and visualized with a Leica DMR bright-field microscope equipped with a digital camera (Leica No. DFC480). Image analysis was performed using FIJI software.
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