β nadph

MDZ was purchased from Sandoz (Tokyo, Japan) as a commercially available injectable solution in a sterilized isotonic buffer (pH 2.8-3.8). It was used without further purification (Hori et al., 2018; (link)Kajikawa et al., 2014) . Two MDZ metabolites, 4-Hydroxy MDZ (4-OH MDZ) and 1'-hydroxy MDZ (1'-OH MDZ), were obtained from BD Biosciences (San Jose, CA, USA) and Cerilliant (Round Rock, TX, USA), respectively. Dexamethasone was from Tokyo Chemical Industry (Tokyo, Japan). β-NADPH was from Oriental Yeast (Tokyo, Japan). Glycerol and dimethyl sulfoxide were purchased from Wako Pure Chemicals (Osaka, Japan). Purified rabbit polyclonal antibody to rat CYP3A23/3A1 (P/N: AB1253) and that to rat CYP3A2 (P/N: AB1276) were from Millipore (Billerica, MA, USA) (Kusaba et al., 2012) (link). The primer pairs for performing polymerase chain reaction (PCR) and Oligo(dT)20 primer for reverse transcription (RT) were obtained via a custom primer synthesis service of Invitrogen/Thermo Fisher Scientific (Waltham, MA, USA). The sequences of the primer pairs are shown in Table 1. RNase inhibitor was obtained from Nacalai Tesque (Kyoto, Japan). All other chemicals were of the finest grade available from local distributers.
[Table 1] -8-