Cells were plated in 6-well plates (1×106 cells/well) and after treatment were trypsinized and analyzed using an Annexin V-fluorescein isothiocyanate detection kit (eBioscience Inc., San Diego, CA, USA). Three concentrations of ZZR (obtained from the result of ZZR Cell Viability assays) and L-OHP (10 µM) were added for 24 h. The samples were immediately processed using flow cytometry to detect the relative amount of Annexin V-FITC-positive-PI-negative cells, as previously described (17 (link)).
Annexin 5 fluorescein isothiocyanate detection kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Annexin V-fluorescein isothiocyanate detection kit is a laboratory reagent used to detect and quantify apoptosis in cell samples. The kit contains Annexin V, a protein that binds to phosphatidylserine, a molecule expressed on the surface of apoptotic cells, and a fluorescent label, fluorescein isothiocyanate (FITC), which allows for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.
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3 protocols using annexin 5 fluorescein isothiocyanate detection kit
1
Apoptosis Assessment of Cancer Cells
2
Annexin V-FITC Apoptosis Assay
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Cells were plated in 6-well plates, and after 24 and 48 h of Dox induction they were trypsinized and analyzed using an annexin V-fluorescein isothiocyanate detection kit (eBioscience Inc., San Diego, CA, USA) according to the manufacturer’s instructions. The samples were immediately processed using a FACSAria III flow cytometer (Becton Dickinson, BD Biosciences) from the Scientific Instrumental Center (University of Granada).
3
Annexin V-FITC Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were plated in six-well plates and after treatment were trypsinized and analyzed using an Annexin V-fluorescein isothiocyanate detection kit (eBioscience Inc., San Diego, CA, USA). The samples were immediately processed using a FACSAria III flow cytometer (Becton Dickinson, BD Biosciences, Franklin Lakes, NJ, USA) from the Scientific Instrumental Center (University of Granada).
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