Methyl palmitate

Methyl laurate (99%), methyl myristate (98%), methyl palmitate (99.5%), methyl stearate (97%), triethylenetetramine (60%) and dimethyl carbonate (99.5%) were purchased from J&K Scientific Ltd. Toluene (99.5%), sodium hydroxide (85%), formic acid (98%), acetic acid (99.5%), lactic acid (85%), absolute ethanol (99.7%), absolute methanol (99.5%) and absolute ethyl ether (99.7%) were provided by Sinopharm Chemical Reagent Beijing Co., Ltd. Acetonitrile (99%) was obtained from an Beijing HWRK Chemical Reagent Co., Ltd. Nutrient broth was supplied by Beijing OBO Biotech Co., Ltd. Benzalkonium chloride solution (50 g l⁻¹) was purchased from Beijing Derun Pharmaceutical Co., Ltd. Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis were obtained from an environmental professional laboratory. The high-purity water (ρ = 18.25 MΩ cm⁻¹) was supplied by an ultrapure laboratory water purification system. N,N,N′,N′-tetramethyl-N,N′-di(2-laurylamideethyl) ethylenediamine methyl carbonate, N,N,N′,N′-tetramethyl-N,N′-di(2-myristylamideethyl) ethylenediamine methyl carbonate and N,N,N′,N′-tetramethyl-N, N′-di(2-palmitylamideethyl) ethylenediamine methyl carbonate (purity greater than 98%) were prepared according to a green method reported in our previous paper [20 (link)].