Supernatants were prepared as described for metabolomics. L-tryptophan (L-Trp) quantification was carried out on an Agilent 1200 HPLC instrument equipped with a Luna 5 μm C18(2) 100 Å column (Phenomenex, 250 mm × 4.6 mm) and coupled with an Agilent LC/MS 6130 Quadrupole analyzer. The mobile phase was composed of 0.1% formic acid in water (v/v) (A) and 0.1% formic acid in acetonitrile (v/v) (B). Linear gradient elution was performed at 0.5 mL/min starting at 1% B increasing to 80% B at 15 min, and finally returning to 1% B at 18 min for column re-equilibration, which was completed at 20 min. Chromatographic data were processed using Agilent OpenLab ChemStation software. The retention time of L-Trp was 11 min, and the wavelength was 225 nm. For the MS detector, data acquisition was performed in single ion monitoring mode [m/z]+ 205 for L-Trp.
Lc ms 6130 quadrupole analyzer
Manufactured by Agilent Technologies
The LC/MS 6130 Quadrupole analyzer is a laboratory instrument designed for the detection and analysis of chemical compounds. It combines liquid chromatography (LC) with mass spectrometry (MS) to provide high-sensitivity and selectivity in identifying and quantifying a wide range of molecules. The core function of this analyzer is to separate, ionize, and detect analytes based on their mass-to-charge ratio.
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2 protocols using lc ms 6130 quadrupole analyzer
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Quantification of L-Tryptophan by HPLC-MS
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Quantification of L-Tryptophan by HPLC-MS
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Supernatants were prepared as described for metabolomics. L-tryptophan (L-Trp) quantification was carried out on an Agilent 1200 HPLC instrument equipped with a Luna 5 μm C18(2) 100 Å column (Phenomenex, 250 mm × 4.6 mm) and coupled with an Agilent LC/MS 6130 Quadrupole analyzer. The mobile phase was composed of 0.1% formic acid in water (v/v) (A) and 0.1% formic acid in acetonitrile (v/v) (B). Linear gradient elution was performed at 0.5 mL/min starting at 1% B increasing to 80% B at 15 min, and finally returning to 1% B at 18 min for column re-equilibration, which was completed at 20 min. Chromatographic data were processed using Agilent OpenLab ChemStation software. The retention time of L-Trp was 11 min, and the wavelength was 225 nm. For the MS detector, data acquisition was performed in single ion monitoring mode [m/z]+ 205 for L-Trp.
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