Seven hundred A549 cells or 1,000 HCT-116 cells in 50 µL of medium were plated per well in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning, Corning, USA). After overnight attachment, cells were dosed with a serial dilution of HQ461 with a D300e digital dispenser (Tecan, Männedorf, Switzerland). Cell survival was measured 72 hr later using CellTiter-Glo luminescent cell viability assay kit (Promega, Madison, USA) following vendor instructions. Luminescence was recorded by EnVison multimode plate reader (PerkinElmer, Waltham, USA). IC50 was determined with GraphPad Prism using baseline correction (by normalizing to DMSO control), the asymmetric (five parameter) equation, and least squares fit.
384 well flat clear bottom white polystyrene tc treated microplates
Manufactured by Corning
Sourced in United States
The 384-well flat clear bottom white polystyrene TC-treated microplates are designed for use in a variety of laboratory applications. The plates feature a 384-well format with a flat clear bottom and are made of white polystyrene material that has been tissue culture-treated to enhance cell attachment. The plates are suitable for various assays and experiments that require a high-throughput format.
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2 protocols using 384 well flat clear bottom white polystyrene tc treated microplates
1
Cytotoxicity Assay of HQ461
2
Dose-dependent cell viability assay
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Seven hundred A549 cells or 1,000 HCT-116 cells in 50 µL of medium were plated per well in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning). After overnight attachment, cells were dosed with a serial dilution of HQ461 with a D300e digital dispenser (Tecan). Cell survival was measured 72 hours later using CellTiter-Glo luminescent cell viability assay kit (Promega) following vendor instructions. Luminescence was recorded by EnVison multimode plate reader (PerkinElmer). IC50 was determined with GraphPad Prism using baseline correction (by normalizing to DMSO control), the asymmetric (five parameter) equation, and least squares fit.
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