Antibodies used are as listed below: mouse monoclonal antibody to α-tubulin (clone B-5-1-2, Sigma, St. Louis, MO) [1:500], mouse monoclonal antibody to neuron specific isoform of β-tubulin (clone TUJ-1, Covance, Berkeley, CA) [1:200], mouse monoclonal antibody to γ-tubulin (clone GTU-88, Sigma) [1:500] and rabbit polyclonal antibody to pericentrin (Covance) [1:500]. Antibody to GCP-WD [1:500] were generated in rabbit by immunizing with recombinant histidine-tagged C-terminal peptide (550–660) that were expressed in Escherichia coli using PET21c vector system (Novagen, Madison, WI).
Clone gtu 88
Manufactured by Merck Group
Sourced in United States
The clone GTU-88 is a laboratory equipment product. It serves as a core function in various research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using clone gtu 88
1
Immunohistochemical Staining of Tubulin
2
Immunoblotting Whole Larval Zebrafish
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Whole larval zebrafish were lysed in 1% Triton X-100/1× PBS by brief sonication. Proteins were resolved by electrophoresis and blotted onto PVDF membranes. We used monoclonal antibodies to detect Trc40 (1:5000; Abcam, Cambridge, MA, USA) and gamma tubulin (1:50,000, clone GTU-88; Sigma-Aldrich Corp.) and a rabbit polyclonal antibody to detect GFP (1:5000; Life Technologies). Immunoreactivity was measured with HRP-conjugated secondary antibodies (Goat anti-mouse HRP 1706516; Bio-Rad Laboratories; and Donkey anti-rabbit HRP, GENA9340v; GE Healthcare Life Sciences, Pittsburg, PA, USA) and chemiluminescent substrate (Femto Glow, Michigan Diagnostics, Royal Oak, MI, USA).
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