β actin mouse mab

Fmoc-amino acids and 2-chlorotrityl chloride resin were purchased from GL Biochem (Shanghai, China). β-Alanine, 4-chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl), crystal violet, bovine serum albumin (BSA), DAPI, propidium iodide (PI), l-phenylalanine and other chemical reagents were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Alkaline phosphatase (ALP) and Lyso-Tracker Red were obtained from Yeasen Biotechnology (Shanghai, China). Dulbecco's modified Eagle's medium (DMEM), RPMI Medium 1640 basic and fetal bovine serum (FBS) were obtained from Gibco (Suzhou, China). Anti-γH2AX (phosphor A139) antibody and goat anti-rabbit IgG H&L (Alexa Fluor® 488) antibody were obtained from Abcam (Shanghai, China). PARP rabbit mAb, cleaved PARP (Asp214) rabbit mAb and caspase 3 rabbit antibody were purchased from Cell Signaling Technology (Shanghai, China). Anti-acetyl histone H3 and anti-acetyl histone H4 (Lys 8) antibodies were provided by Millipore (Billerica, USA). β-Actin Mouse mAb and peroxidase-conjugated affinipure goat anti-mouse/rabbit IgG (H + L) were bought from Proteintech Group Inc. (Wuhan, China). FITC Annexin V apoptosis detection kit was purchased from BD Pharmingen (San Diego, CA, USA). Dynasore was purchased from TCI Development Co., Ltd. (Shanghai, China).

Sprague-Dawley (SD) rats were selected for all experiments, which were provided by the China National Institute for Food and Drug Control. Fifteen male SD rats (40–60 g) were used to extract BMMSCs, and 24 male SD rats (200–220 g) were used to acquire donor livers. This study was approved by the Ethics Committee of Tianjin first Central Hospital (Permit number: 2016-03-A1).
Laboratory reagents: Adenoviruses expressing the green fluorescence protein (GFP-Adv) of rats (Shanghai Gene Chem Co, Shanghai, China); Microtubule-associated protein 1 light chain 3 (LC3)-I/II rabbit mAb (Cell Signaling Technology, Boston, MA, USA); Prostaglandin-endoperoxide synthase 2 (PTGS2) rabbit antibody, P62 rabbit antibody, rabbit nuclear receptor co-activator 4 (NCOA4) pAb, and β-actin mouse mAb (Proteintech Inc., Rosemont, IL, USA); Rabbit Anti-glutathione peroxidase 4 (GPX4) antibody (BIOSS, Beijing, China); Rabbit Ferritin Heavy Chain (FTH1) mAb (Abcam, Cambridge, Cambridge, UK); Malondialdehyde (MDA) assay kit and Glutathione (GSH) assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

We used the following antibodies: β-actin mouse mAb, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mouse mAb, β-tubulin mouse mAb, Rab7A rabbit mAb, Rab7–interacting lysosomal protein (RILP) rabbit mAb, CD63 rabbit mAb, goat anti-rabbit IgG horseradish peroxidase (HRP) conjugate mAb, goat anti-mouse IgG HRP conjugate mAb, heavy chain of rabbit IgG mAb, mouse anti-rabbit IgG light chain specific HRP conjugate mAb (Proteintech, Rosemont, IL), Vps34/PI3KC3 rabbit mAb, Rab11 rabbit mAb, Lamp1 rabbit mAb, LC3A/B rabbit mAb, matrix metalloproteinase 2 (MMP-2) rabbit mAb, epidermal growth factor receptor (EGFR) rabbit mAb, p-EGFR (Y1068) rabbit mAb (Cell Signaling Technology, Danvers, MA), Lamp1 mouse mAb, MAPLC3β mouse mAb (Santa Cruz Biotechnology, Dallas, TX), TSG101 rabbit mAb (Abcam, Cambridge, MA), EGFR mouse mAb (Bioss, Woburn, MA), AF594 AffiniPure goat anti-rabbit, AF647 AffiniPure goat anti-mouse, AF594, AffiniPure donkey anti-rabbit, DyLight 488 horse anti-mouse (Vector Laboratories, Burlingame, CA). Reagents we used in this study were listed: Vps34-IN-1 (MedChemExpress, Monmouth Junction, NJ), CQ (Sigma-Aldrich, St. Louis, MO), human epidermal growth factor (EGF; Sigma-Aldrich).