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Rabbit monoclonal anti p cofilin antibody

Manufactured by Cell Signaling Technology
Sourced in United States

The Rabbit monoclonal anti-p-cofilin antibody is a laboratory reagent used to detect and study the phosphorylated form of the cofilin protein. Cofilin is an actin-binding protein that plays a crucial role in regulating the dynamics of the actin cytoskeleton.

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2 protocols using rabbit monoclonal anti p cofilin antibody

1

CDC42 and N-WASP Signaling Pathway

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Primary antibodies: a rabbit polyclonal anti-CDC42 antibody and rabbit monoclonal anti-N-WASP antibody were purchased from Abcam (Cambridge, UK); a rabbit polyclonal anti-ROCK antibody was purchased from Santa Cruz (Santa Cruz, CA, USA); a rabbit monoclonal anti-p-cofilin antibody and a rabbit polyclonal anti-α-tubulin antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Secondary antibodies: goat anti-rabbit IgG/FITC and goat anti-rabbit IgG/TRITC were purchased from Zhongshan Golden Bridge Biotechnology (Beijing, China); HRP-conjugated Pierce goat anti-rabbit IgG was purchased from Cell Signaling Technology. Chemicals: all chemicals used in this study were obtained from Sigma Chemical Company (St. Louis, MO, USA), unless otherwise specified, and ML141, a potent, selective inhibitor of CDC42, was purchased from Santa Cruz.
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2

Immunoblotting Assay for PKD1 and MAPK

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Rabbit polyclonal anti-PKD1 antibody (#SAB4502371), mouse monoclonal anti-α-tubulin-FITC (F2168) antibody and Phalloidin-Atto 590 (93042) were purchased from Sigma (St. Louis, MO, USA). Rabbit monoclonal anti-PKD antibody (#2052), rabbit polyclonal anti-phospho-MAPK antibody (#4370), rabbit monoclonal anti-p-cofilin antibody (#3313), mouse monoclonal anti-β-actin antibody (#3700), rabbit monoclonal anti-GAPDH antibody (#2118) were purchased from Cell Signaling Technology (Danvers, MA, USA). CID755673 was from Selleck (Houston, TX, USA). All other chemicals were purchased from Sigma (St. Louis, MO, USA), unless otherwise stated.
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