Anti igg4

Manufactured by Abcam

Anti-IgG4 is a laboratory reagent used for the detection and measurement of immunoglobulin G subclass 4 (IgG4) in various biological samples. It functions as a specific antibody that binds to IgG4 molecules, allowing for their identification and quantification.

Automatically generated - may contain errors

Lab products found in correlation

Superpicture polymer detection kit, by Thermo Fisher Scientific (2 mentions) Anti il 4, by R&D Systems (2 mentions) Anti icos, by Cell Signaling Technology (2 mentions) Bcl 6, by Biocare Medical (2 mentions) Opal 3 plex kit, by Thermo Fisher Scientific (2 mentions) Cxcr5, by R&D Systems (2 mentions) Prolong gold antifade mountant containing dapi, by Thermo Fisher Scientific (2 mentions) Anti aid, by Thermo Fisher Scientific (2 mentions) Gata3, by Biocare Medical (2 mentions)

2 protocols using anti igg4

Multimodal Immunophenotyping of Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue samples were fixed in formalin, embedded in paraffin, and sectioned. These specimens were incubated with the following antibodies: anti-AID (clone: ZA001; Invitrogen), anti-IgG4 (clone: ab109493; Abcam), anti-ICOS (clone: 89601; Cell Signaling Technology), anti-IL-4 (clone: MAB304; R&D Systems), GATA3 (clone: CM405A; Biocare), CXCR5 (clone: MAB190; R&D Systems), Bcl-6 (clone: CM410A,C; Biocare), BATF (clone: 10538; Cell Signaling Technology), CD4 (clone: CM153A; Biocare), and CD19 (clone: CM310 A,B; Biocare), followed by incubation with secondary antibodies using a SuperPicTure Polymer Detection Kit (Invitrogen) and an Opal 3-Plex Kit (Fluorescein, Cyanine3, and Cyanine5). The samples were mounted with ProLong Gold Antifade mountant containing DAPI (Invitrogen).

Maehara T., Mattoo H., Mahajan V.S., Murphy S.J., Yuen G.J., Ishiguro N., Ohta M., Moriyama M., Saeki T., Yamamoto H., Yamauchi M., Daccache J., Kiyoshima T., Nakamura S., Stone J.H, & Pillai S. (2018). The expansion in lymphoid organs of IL-4+ BATF+ T follicular helper cells is linked to IgG4 class switching in vivo. Life Science Alliance, 1(1), e201800050.

+ Open protocol

+ Expand

Multiparameter Immunohistochemistry of Immune Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue samples were fixed in formalin, embedded in paraffin, and sectioned. These specimens were incubated with the following antibodies: anti-AID (clone: ZA001; Invitrogen), anti-IgG4 (clone: ab109493; Abcam), anti-ICOS (clone: 89601; Cell Signaling Technology), anti-IL-4 (clone: MAB304; R&D Systems), GATA3 (clone: CM405A; Biocare), CXCR5 (clone: MAB190; R&D Systems), Bcl-6 (clone: CM410A, C; Biocare), BATF (clone: 10538; Cell Signaling Technology), CD4 (clone: CM153A; Biocare), and CD19 (clone: CM310 A, B; Biocare), followed by incubation with secondary antibodies using a SuperPicTure Polymer Detection Kit (Invitrogen) and an Opal 3-Plex Kit (Fluorescein, Cyanine3, and Cyanine5). The samples were mounted with ProLong Gold Antifade mountant containing DAPI (Invitrogen).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!