Total protein was extracted using the radio immunoprecipitation assay (RIPA) buffer (Solarbio, Beijing, China) supplemented with phenylmethanesulfonyl fluoride (PMSF) and a phosphatase inhibitor (CWBIO, China), and protein was quantified using a BCA kit (Bicinchoninic Acid Assay Kit, Beyotime, China). The proteins were then separated by 8% or 10% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Massachusetts, USA). The PVDF membranes were blocked with tris buffered saline with tween-20 (TBST) containing 5% (bovine serum albumin) BSA (Solarbio, Beijing, China). The membranes were incubated overnight at 4°C with the following primary antibodies: anti-CD206 (EPR22489-7), anti-TSG101 (Ab125011), anti-CD9 (Ab236630), and anti-STAT3 (Ab68153) from Abcam (Massachusetts, USA), anti-phospho-STAT3 (Tyr705) (#AF3293) from Affinity (Jiangsu, China), anti-ZO-1 (No. 21773–1-AP), anti-ZEB1 (No. 21544–1-AP), anti-α-SMA (No. 80008–1-RR), and anti-E-cadherin (No. 20874–1-AP) from Proteintech (Wuhan, China). The membrane was incubated with the secondary antibody for 1 hour at room temperature. The bands were visualized using a Chemiluminescence Kit (Millipore, USA).
Anti e cadherin no 20874 1 ap
Manufactured by Proteintech
Sourced in China
Anti-E-cadherin (No. 20874–1-AP) is a primary antibody that recognizes the E-cadherin protein. E-cadherin is a cell-cell adhesion molecule that plays a critical role in maintaining the integrity of epithelial tissues. This antibody can be used to detect and study the expression of E-cadherin in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Merck Group (2 mentions)
Chemiluminescence kit, by Merck Group (1 mentions)
A phosphatase inhibitor, by Beyotime (1 mentions)
Pvdf membrane, by Solarbio (1 mentions)
Anti stat3 ab68153, by Abcam (1 mentions)
Ripa buffer, by Solarbio (1 mentions)
Bovine serum albumin (bsa), by Solarbio (1 mentions)
Chemiluminescence kit, by Absin (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)
2 protocols using anti e cadherin no 20874 1 ap
1
Comprehensive Protein Analysis: RIPA, SDS-PAGE, and Western Blotting
2
Protein Extraction and Western Blot Analysis
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Total protein was extracted with RIPA buffer containing PMSF (Solarbio, Beijing, China) and quantified with a BCA kit. Then, the proteins were separated on SDS-PAGE gels and transferred to PVDF membranes (Millipore, Massachusetts, USA). The PVDF membranes were blocked with TBST containing 5% skim milk powder and incubated with anti-YAP (No. 14074), anti-phospho-YAP (Ser127) (No. 13008), and anti-SMAD3 (No. 9523) antibodies from Cell Signaling Technology (MA, USA) and anti-ZO-1 (No. 21773-1-AP), anti-ZEB1 (No. 21544-1-AP), and anti-E-cadherin (No. 20874-1-AP) antibodies from Proteintech (Wuhan, China) at 4 °C overnight. Secondary antibodies were hybridized with the membranes at room temperature for 1 h. A chemiluminescence kit (Absin, Shanghai, China) was used to visualize the membrane.
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