Live dead assay kit

Cell viability of MSC in TEL was measured with a Live/Dead assay kit (Best-Bio, Shanghai, China). Experiments were performed according to the manufacturer's instructions. The representative images were obtained using a ZEISS confocal fluorescence microscope (ZEISS, Jena, Germany).

Cell viability of MSC in TEL was measured with a Live/Dead assay kit (Best-Bio, Shanghai, China). Experiments were performed according to the manufacturer’s instructions. The representative images were obtained using a ZEISS confocal fluorescence microscope (ZEISS, Jena, Germany).

To investigate the role of SIRT6 in migration, phagocytosis and polarization of macrophages, the SIRT6 selective inhibitor OSS_128167 (Cat#HY-107454, MedChemExpress, USA) was used to suppress the activity of SIRT6 [39 (link)]. And to assess the potential cytotoxicity of OSS_128167, Calcein-AM/ Propidium Iodide (PI) staining assay was performed by using a Live/Dead assay kit (Cat#BB-4126, BestBio, Shanghai, China). Briefly, BMDM were seeded at 2 × 10⁴ per well in 24-well plates and incubated overnight to allow cell adherence. Then treated with 200 μM of SIRT6 inhibitor OSS_128167 for 24 h based on the previous studies [39 (link), 40 (link)], and the BMDM treated with 0.1‰ DMSO was assigned as the Vehicle group. The cells were washed once with sterile PBS and incubated in 0.4 μM fluorescent green Calcein-AM for 30 min at 37 ℃ to check live cells and 2 μM fluorescent red PI for 5 min at 37 ℃ for dead cells.