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Mouse trem2

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Mouse TREM2 is a recombinant protein that represents the extracellular domain of the mouse Triggering Receptor Expressed on Myeloid cells 2 (TREM2) protein.

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Lab products found in correlation

Anti his c term hrp, by Thermo Fisher Scientific (2 mentions) Mca4772el, by Bio-Rad (2 mentions) Rat igg1, by Bio-Rad (2 mentions) Nicastrin, by Santa Cruz Biotechnology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Ddk flag, by OriGene (2 mentions)

Spelling variants of this product

TREM2 (8 citations) APC anti-human/mouse Trem2 (2 citations)

2 protocols using mouse trem2

1

Antibody Validation for Protein Detection

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The following antibodies were used: mouse TREM2 (#AF1729, targeting on N-terminal TREM2, 1:500 for western blot; R&D); DDK (FLAG) (#TA50011, 1:2000 for western blot; Origene); V5 (#46-0705, 1:2000 for western blot; Life Technologies, Burlington); Nicastrin (#sc-14369, 1:200 for western blot; Santa Cruz); GAPDH (#2118, 1:3000 for western blot; Cell Signaling); Anti-His (C-term)-HRP (#46-0707, 1:2000 for dot blot, Invitrogen); Aβ (6E10, 1:1000 for western blot and dot blot, Covance); TrueBlot secondary antibodies conjugated with horseradish peroxidase (anti-goat IgG, anti-mouse IgG, and anti-sheep IgG, 1:1000 for western blot after immunoprecipitation; Rockland); secondary antibodies conjugated with horseradish peroxidase (anti-mouse IgG; anti-rabbit IgG, 1:2000 for western blot; Thermo Scientific). Function-blocking TREM2 antibody (Clone 78.18, 25 μg/ml for blocking assay, AbD Serotec catalogue number MCA4772EL) and a control antibody (Rat IgG1, AbD Serotec) were used for blocking experiments.
Vilalta A., Zhou Y., Sevalle J., Griffin J.K., Satoh K., Allendorf D.H., De S., Puigdellívol M., Bruzas A., Burguillos M.A., Dodd R.B., Chen F., Zhang Y., Flagmeier P., Needham L.M., Enomoto M., Qamar S., Henderson J., Walter J., Fraser P.E., Klenerman D., Lee S.F., St George-Hyslop P, & Brown G.C. (2021). Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation. The Journal of Biological Chemistry, 296, 100631.
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2

Antibody Characterization for TREM2 Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: human TREM2 (#AF1828, 1:100 for immunocytochemistry (ICC); 1:1000 for western blot; R&D); mouse TREM2 (#AF1729, 1:500 for western blot; R&D); DDK (FLAG) (#TA50011, 1:2000 for western blot; Origene); V5 (#46-0705, 1:2000 for western blot; Life Technologies, Burlington); Nicastrin (#sc-14369, 1:200 for western blot; Santa Cruz); GAPDH (#2118, 1:3000 for western blot; Cell Signaling); Anti-His (C-term)-HRP (#46-0707, 1:2000 for dot blot, Invitrogen); Aβ (6E10, 1:1000 for western blot and dot blot, Covance); TrueBlot secondary antibodies conjugated with horseradish peroxidase (anti-goat IgG, anti-mouse IgG and anti-sheep IgG, 1:1000 for western blot after immunoprecipitation; Rockland); Secondary antibodies conjugated with horseradish peroxidase (anti-mouse IgG; antirabbit IgG, 1:2000 for western blot; Thermo Scientific). Function-blocking TREM2 antibody (Clone 78.18, 25 μg/ml for blocking assay, AbD Serotec catalogue number MCA4772EL) and a control antibody (Rat IgG1, AbD Serotec) were used for blocking experiments.
Vilalta A., Zhou Y., Sévalle J., Griffin J.K., Satoh K., Allendorf D.H., De S., Puigdellívol M., Bruzas A., Burguillos M.Á., Dodd R.B., Chen F., Zhang Y., Flagmeier P., Needham L., Enomoto M., Qamar S., Henderson J.W., Walter J., Fraser P.E., Klenerman D., Lee S.F., George‐Hyslop P.S., & Brown G.C. (2020). Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, while the Alzheimer’s R47H mutant does the opposite.
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