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Mj thermocycler

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The MJ thermocycler is a laboratory instrument designed for performing polymerase chain reaction (PCR) experiments. It accurately controls the temperature of samples to facilitate DNA amplification and other temperature-dependent processes. The MJ thermocycler provides precise temperature regulation and cycling capabilities to support various molecular biology applications.

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Lab products found in correlation

Taq dna polymerase, by Promega (2 mentions) 25 bp dna ladder, by Promega (2 mentions) Gel pro analyser v3, by Media Cybernetics (2 mentions) Phusion master mix, by New England Biolabs (1 mentions) Veriti thermocycler, by Thermo Fisher Scientific (1 mentions) Qubit instrument, by Thermo Fisher Scientific (1 mentions) Bioanalyzer, by Agilent Technologies (1 mentions) Amppure xp beads, by Beckman Coulter (1 mentions)

Spelling variants of this product

Thermocycler (392 citations) MJ Mini thermocycler (18 citations) MJ Research PTC-200 thermocycler (7 citations) MJ Mini Personal Thermocycler (5 citations) MJ Thermocycler PTC-200 (2 citations)

4 protocols using mj thermocycler

1

PCR Amplification and Gel Electrophoresis

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The amplification reactions (PCR) were performed in a total volume of 20 ll, containing 25 ng of genomic DNA as template, 50 pmol of each of the primers, 200 ll dNTPs, 2 mM MgCl 2 , 1 X of Taq polymerase buffer and 1 U Taq DNA polymerase (Promega Madison, WI, USA). Amplification was carried out in 96 well plates by means of the MJ thermocycler (PTC-100 MJ Research, Watertown, Mass., USA) with an initial cycle denaturation of 2 min at 94 °C, 35 cycles with a second step of 15 s at 92 °C, a third step of 15 s at the binding temperature of the primers (Supplementary material S3), and 30 s at 72 °C to extend the fragment. It was finished with a 5 min extension at 72 °C, keeping the extension product at 16 °C.
All amplification products and negative tests were checked by means of a 1% agarose gel electrophoresis in TBE buffer. Electrophoresis in denaturing polyacrylamide gels (6% p/v) was performed and silverstained as detailed by Benbouza et al. (2006) . Band size was determined by comparison with 25 bp DNA ladder (Promega, USA) employing Gel Pro Analyser V3.1 (Media Cybernetics, USA).
Amado Cattáneo R., McCarthy A.N, & Feingold S.E. (2020). Evidence of genetic diversity within Solanum Lycopersicum L. ‘Platense’ landrace and identification of various subpopulations. Genetic resources and crop evolution, 67(8).
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2

PCR Amplification and Gel Electrophoresis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The amplification reactions (PCR) were performed in a total volume of 20 ll, containing 25 ng of genomic DNA as template, 50 pmol of each of the primers, 200 ll dNTPs, 2 mM MgCl 2 , 1 X of Taq polymerase buffer and 1 U Taq DNA polymerase (Promega Madison, WI, USA). Amplification was carried out in 96 well plates by means of the MJ thermocycler (PTC-100 MJ Research, Watertown, Mass., USA) with an initial cycle denaturation of 2 min at 94 °C, 35 cycles with a second step of 15 s at 92 °C, a third step of 15 s at the binding temperature of the primers (Supplementary material S3), and 30 s at 72 °C to extend the fragment. It was finished with a 5 min extension at 72 °C, keeping the extension product at 16 °C.
All amplification products and negative tests were checked by means of a 1% agarose gel electrophoresis in TBE buffer. Electrophoresis in denaturing polyacrylamide gels (6% p/v) was performed and silverstained as detailed by Benbouza et al. (2006) . Band size was determined by comparison with 25 bp DNA ladder (Promega, USA) employing Gel Pro Analyser V3.1 (Media Cybernetics, USA).
Cattáneo R.A., McCarthy A.N., & Feingold S.E. (2020). Evidence of genetic diversity within Solanum Lycopersicum L. ‘Platense’ landrace and identification of various subpopulations. Genetic Resources and Crop Evolution, 67(8), 2057-2069.
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3

Optimized cfDNA PCR Amplification and Purification

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We used 16 μL of eluted cfDNA as the template for the PCR amplification. Thus, the overall plasma equivalent per PCR was 1.07 mL. The PCR was performed on an MJ Thermocycler (BioRad, Hercules, CA, USA) or a Veriti Thermocycler (Applied Biosystems, Waltham, MA, USA) in a PCR with a total volume of 40 μL with 0.5 μM of each primer (Eurofins Genomics, Ebersberg, Germany), 1× Phusion master mix (New England Biolabs, Ipswich, MA, USA), and with the following thermoprofile: one cycle of 98°C 30 s, 40 cycles of 98°C 15 s, 63°C 15 s, 72°C 20 s, finishing with 72°C 5 min, 4°C hold. We routinely setup 2 PCR reactions and pool the volumes before the bead purification.
The PCR products were purified with AMPpure XP beads (Beckman Coulter, Brea, CA, USA), and the concentration after purification was measured on a Qubit instrument (ThermoFischer, Waltham, MA, USA), both procedures performed according to the recommendations of the manufacturer. Before proceeding to sequencing, the concentration of purified PCR product was required to be more than 5 μg/mL. Also, the PCR products were tested on a Bioanalyzer (Agilent, Santa Clara, CA, USA) to ascertain if spurious amplification had occurred. More than 90% of the PCR product was required to be in a single peak to proceed with sequencing. If these criteria were not met, a novel amplification was undertaken.
Rieneck K., Clausen F.B., Bergholt T., Nørgaard L.N, & Dziegiel M.H. (2022). Non-Invasive Fetal K Status Prediction: 7 Years of Experience. Transfusion Medicine and Hemotherapy, 49(4), 240-249.
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4

Semiquantitative RT-PCR Analysis of SSTR and DR2 Genes

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In a subset of 20 LCs, the five SSTRs and DR2 gene transcripts were analyzed using semiquantitative RT-PCR. Total RNA extraction and subsequent cDNA synthesis with reverse transcription were performed using standard techniques. Amplification of cDNAs was achieved using ready-to-use Long-Range PCR Nucleotide Mix (Roche Diagnostics Corp., Indianapolis, Ind., USA) and appropriate temperature programs on a MJ Thermocycler (Bio-Rad). The data were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) levels which served as internal control. The primer sequences for SSTR1, SSTR2A, SSTR3, SSTR4, SSTR5, DR2, and GAPDH (control gene) are listed in online supplementary table 1 (see www.karger.com/doi/10.1159/000381061).
Kanakis G., Grimelius L., Spathis A., Tringidou R., Rassidakis G.Z., Öberg K., Kaltsas G, & Tsolakis A.V. (2015). Expression of Somatostatin Receptors 1-5 and Dopamine Receptor 2 in Lung Carcinoids: Implications for a Therapeutic Role. Neuroendocrinology, 101(3).
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