Alizarin Red S powder, formaldehyde solution, antibiotic-antimycotic solution, decalcifying solution, and cetylpyridinium chloride were obtained from Sigma-Aldrich (St. Louis, MO, USA). α-MEM medium and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Rat osteoblast differentiation medium was provided by Cell Applications (San Diego, CA, USA). Phosphate-buffered saline (PBS) and 0.25 % trypsin-EDTA solution were purchased from Welgene (Daegu, Korea). Primary antibodies against osterix, alkaline phosphatase (ALP), and GAPDH were purchased from Abcam (Cambridge, UK). Type-1 collagen and FGF2 were obtained from Novus Biologicals (Centennial, CO, USA). Antibodies to P-Met, c-Met, and Runx-2 were provided by Cell Signaling Technology (Danvers, MA, USA). The osteocalcin antibody was obtained from Bioss (Woburn, MA, USA). Hydroxyapatite/beta-tricalcium phosphate (HA/β-TCP) ceramic powder was purchased from Biomatlant (Vigneux, France). Rat HGF ELISA Kit, Rat FGF2 ELISA Kit and recombinant FGF2 protein were obtained from R&D Systems (Minneapolis, MN, USA). The rat VEGF enzyme-linked immunosorbent assay (ELISA) kit was purchased from Abclonal (Woburn, MA, USA). Rat SDF-1α ELISA Kit was provided by Biorbyt (Cambridge, UK). Recombinant rat HGF protein was purchased from LifeSpan BioSciences (Seattle, WA, USA).
Rat osteoblast differentiation medium
Manufactured by Cell Applications
Sourced in United States
Rat osteoblast differentiation medium is a cell culture medium designed to support the differentiation of rat osteoblasts, which are bone-forming cells. The medium provides the necessary nutrients and growth factors to promote the development of mature osteoblasts from progenitor cells.
Automatically generated - may contain errors
Lab products found in correlation
α mem medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Formaldehyde solution, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Welgene (1 mentions)
Cetylpyridinium chloride, by Merck Group (1 mentions)
Trypsin edta solution, by Welgene (1 mentions)
Decalcifying solution, by Merck Group (1 mentions)
Antibiotic antimycotic solution, by Merck Group (1 mentions)
Alizarin red s powder, by Merck Group (1 mentions)
Rat fgf 2 elisa kit, by R&D Systems (1 mentions)
Ab8448, by Abcam (1 mentions)
Ab150077, by Abcam (1 mentions)
2 protocols using rat osteoblast differentiation medium
1
In Vitro Osteoblast Differentiation
2
Stem Cell-Assisted Furcation Lesion Repair in Rats
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Four groups of materials were used for animal transplantation in the treatment of furcation lesions in rats: T1 (DAM, only membrane), T2 (DAM+ASCs), T3 (DAM+ECM), and T4 (DAM+ECM+ASCs). ASCs 2.5 × 104 cells/cm2 were cultivated over the 8 mm diameter DAM disks and stretched onto 12-well plates. Four days before surgery, cells were associated with DAM in treatment groups T2 and T4. Preparation of DAM with ECM (T3 and T4) required cell culture and osteogenic differentiation in advance to simultaneously heal the different groups. Disks of DAM were associated initially with 2.5 × 104 cells/cm2 ASCs and a regular cell culture medium for cell proliferation. After cell semiconfluency observation, osteogenic differentiation of ASCs was induced with Rat Osteoblast Differentiation Medium (Cell Applications Inc., San Diego, CA, USA) for four weeks.
Cell and extracellular matrix distribution was observed after 2.5% glutaraldehyde fixation with histochemical staining with Giemsa and Alizarin red, respectively. Primary antibodies to Osteopontin (OPN, Rabbit polyclonal anti osteopontin ab8448, ABCAM, Cambridge, UK), followed by Alexa Fluor 488-conjugated secondary antibodies (Goat anti-rabbit IgG ab 150077, ABCAM, Cambridge, UK) were used for protein immunolabeling and Hoechst H3569 (Invitrogen, Eugene, OR, USA) for nuclear staining.
Cell and extracellular matrix distribution was observed after 2.5% glutaraldehyde fixation with histochemical staining with Giemsa and Alizarin red, respectively. Primary antibodies to Osteopontin (OPN, Rabbit polyclonal anti osteopontin ab8448, ABCAM, Cambridge, UK), followed by Alexa Fluor 488-conjugated secondary antibodies (Goat anti-rabbit IgG ab 150077, ABCAM, Cambridge, UK) were used for protein immunolabeling and Hoechst H3569 (Invitrogen, Eugene, OR, USA) for nuclear staining.
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