Zolazepam hcl

During January–March 2011–2014, we captured white‐tailed deer using Clover traps (Clover, 1956 ), drop nets (Ramsey, 1968 (link)), rocket‐propelled nets (Hawkins et al., 1968 ), and free‐darting techniques. We chemically immobilized captured deer with a mixture of Telazol HCl (tiletamine HCl, 2 mg/kg, and zolazepam HCl, 4 mg/kg, Pfizer Animal Health) and Rompun (xylazine HCl, 2 mg/kg). We aged deer by examining tooth‐wear and development (Severinghaus, 1949 (link)), and fit an Iridium‐satellite uplink Global Positioning System (GPS) telemetry collar (Advanced Telemetry Systems, Inc) to all deer that were <22 months of age. We targeted juveniles (<12 months) and subadults (≥12 and <22 months) because these age classes are the most likely to disperse (Diefenbach et al., 2008 (link); Nelson & Mech, 1984 (link); Nixon et al., 1991 ), and we assumed these young deer were captured in/nearby their natal home range. After workup, we administered tolazoline (100 mg/kg) to aid in recovery from immobilization. All deer capture and handling methodology was approved by the Southern Illinois University Carbondale Institutional Animal Care and Use Committee (protocol #10–032), and followed guidelines set forth by the American Society of Mammalogists for the use of wild mammals in research (Sikes et al., 2011 (link)).

On d8 of the study (postnatal day [PND] 10), blood was collected via jugular vein in either plain, K₂EDTA, or Na Citrate-laced vacuum tubes (BD Biosciences, Franklin Lakes, NJ) to perform chemistry, complete blood count (CBC), and coagulation time analyses, respectively. On d22, animals were sedated with an intramuscular injection of Telazol^® (Tiletamine HCl and Zolazepam HCl, 3.5 mg/kg BW each, Pfizer Animal Health, Fort Dodge, IA) and blood was collected via cardiac puncture for chemistries, CBC, and coagulation time analyses 3–6 h after the final feeding. Piglets were then euthanized by an intravenous injection of 86 mg/kg BW sodium pentobarbital (Euthasol; Virbac AH, Inc., Fort Worth, TX). Urine samples for urinalysis, spleen, stomach, kidneys, heart, lungs, intestine, and liver were collected and fixed for analysis as previously described [15 (link)].