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Tris buffer

Manufactured by SD Fine Chemicals
Sourced in India

Tris buffer is a widely used buffering agent in biochemical and molecular biology applications. It is an aqueous solution that maintains a stable pH range, typically between 7.0 and 9.0, making it suitable for various laboratory procedures. The core function of Tris buffer is to provide a controlled and consistent pH environment for the efficient functioning of enzymes, proteins, and other biomolecules during experiments and analyses.

Automatically generated - may contain errors

2 protocols using tris buffer

1

Spectroscopic Characterization of Donor-Acceptor Complex

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The donor 2-methyl-8-quinolinol (2 MQ), acceptor chloranilic acid (CHLA), and CT-DNA were acquired from Sigma-Aldrich USA (98%). Sodium chloride and tris buffer (99.5%) were procured from SD Fine Chemicals Limited. The solvents acetonitrile (Finar 99.5%), methanol (Finar 99.5%), and ethanol (Hayman 100%) were used at a high analytical grade. Safety glasses, hand covers, and dust masks were used during all experiments.
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2

Synthesis of Iron Oxide Nanoparticles

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For the synthesis of iron oxide nanoparticles, ferrous sulphate heptahydrate (FeSO4.7H2O) was purchased from HiMedia Laboratories, India. Ferric chloride hexahydrate (FeCl3.6H2O) was purchased from Thomas Baker (Chemicals) Pvt. Ltd., India. Tetramethyl ammonium hydroxide (C4H13NO), formaldehyde (CH2O), acetone (C3H6O), Glucose, EDTA, NaOH and Potassium Acetate, Tris Buffer, and starch were purchased from SD Finechem Limited, India. Polyethyleneimine was procured from TCI CO., Ltd. N, N Dimethylformamide was purchased from Fisher Scientific, Pvt., Ltd. For proper solution mixing during the incubation, a cyclo-rotator was designed to fit our purpose. HeLa cells were obtained from Stellixer Biotec, Bengaluru, India.
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