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2 protocols using monopotassium phosphate

1

Antioxidant Activity Determination Protocol

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DHA (98%), guaiacol (98%) and 2-thiobarbituric acid (98%) were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). Anhydrous ethanol was obtained from Zhengzhou Paini Chemical Reagent Factory (Zhengzhou, China). Tween-80 was obtained from Tianjin Baishi Chemical Co., Ltd (Tianjin, China). Propidium iodide (95%) was purchased from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). Hydrogen peroxide (H 2 O 2 , 30%) was purchased from Xilong Chemical Co., Ltd (Guangdong, China). Dipotassium hydrogen phosphate and monopotassium phosphate were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). The active oxygen detection kit was purchased from Beyotime Biotechnology Research Institute (Shanghai, China). PDB medium was produced from Qingdao Hi-Tech Industrial Park Haibo Biotechnology Co., Ltd.
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2

Quantitative Analysis of Cyanogenic Glycosides

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Standards of linamarin (98%) and lotaustralin (97%) were obtained from TRC (Toronto, Canada). Cyanogen in water (GBW(E) 080115) at 50 µg/mL was produced by the Chinese Academy of Metrology. Acetonitrile and methanol (HPLC grade) were from Sigma-Aldrich (Steinheim, Germany). Chloramine-T, isonicotinic acid, barbituric acid and β-glucosidase were obtained from Sigma-Aldrich (Shanghai, China). All other reagents including sodium hydroxide, monopotassium phosphate, disodium hydrogen phosphate were of analytical grade purchased from Sinopharm Co., Ltd. (Shanghai, China). Ultrapure water used in the experiments was prepared by Milli-Q purification system (Millipore, Bedford, USA).
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