The isolated mantle was pinned to a Silgard-coated dissection dish, and the cutting edge of the nerve bundle from the right parietal nerve was physically isolated in a Vaserin dam filled with 5% Lucifer Yellow solution. The preparation was incubated at 20°C for several days to allow the dye to spread throughout the right parietal nerve, followed by fixation with 4% paraformaldehyde in 0.1 M PB solution and examination under a fluorescence microscope (Optiphoto-2, Nikon, Tokyo, Japan). The fixed mantle was cut into 50 μm thick sections and immunohistologically examined through staining with anti-Lucifer Yellow antibody (A-5750, Molecular Probe, Eugene, OR, USA) as described above. Since we used anti-Lucifer Yellow antibody obtained from rabbit IgG, we used biotinylated anti-rabbit IgG and visualized with ABC complex as described above. The stained images were examined on glass slides under a microscope (Diaphoto-TMD, Nikon, Tokyo, Japan) and captured using a digital camera (EOS kiss X2, Canon, Tokyo, Japan).
Eos kiss x2
Manufactured by Canon
Sourced in Japan
The Canon EOS kiss X2 is a digital single-lens reflex (DSLR) camera. It features a 22.2-megapixel APS-C CMOS sensor and DIGIC 4 image processor. The camera supports full HD 1080p video recording at 30 frames per second. It has a 3-inch LCD monitor and an optical viewfinder. The EOS kiss X2 is compatible with a range of Canon EF and EF-S lenses.
Automatically generated - may contain errors
Lab products found in correlation
A5750, by Thermo Fisher Scientific (1 mentions)
Fv1200, by Olympus (1 mentions)
Dm5000b, by Leica (1 mentions)
S8ap0, by Leica (1 mentions)
Dfc500, by Leica (1 mentions)
Jfc 1200, by JEOL (1 mentions)
Smz1500, by Nikon (1 mentions)
Jsm 6510lv, by JEOL (1 mentions)
Jcpd 5, by JEOL (1 mentions)
Axio imager m2, by Zeiss (1 mentions)
3 protocols using eos kiss x2
1
Lucifer Yellow Nerve Tracing
2
Seedling Microscopy Imaging Techniques
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Seedlings were observed by using stereoscopic microscopes S8AP0 (Leica Microsystems, http://www.leica-microsystems.com/ ) equipped with a CCD camera (DFC500, Leica) or SMZ1500 (Nikon Instruments, http://www.nikoninstruments.com/ ) equipped with a digital camera (EOS Kiss X2, Canon, http://www.canon.jp/ ). Differential interference contrast (DIC) microscopy was conducted by DM5000B (Leica) equipped with CCD camera (DFC500). Confocal imaging was conducted with confocal laser scanning microscopes, FV-1200 (Olympus, http://www.olympus-lifescience.com/ ) or C1 (Nikon). Images were processed by Image-J software. The quantification of cortical microtubules was conducted according to ref. 7 (link).
In scanning electron microscopy (SEM), plants were fixed in a solution of 1% glutaraldehyde in 50 mM sodium phosphate buffer for overnight, dehydrated in the ethanol series (30, 60, 80, 90, and 100%) and then transferred to a solution of isoamyl acetate. Samples were dried by a critical point dryer (JCPD-5, JEOL,http://www.jeol.co.jp/en/ ) using liquid CO2, coated with gold by a ion sputter (JFC-1200, JEOL) and observed with a scanning electron microscope (JSM-6510LV, JEOL).
In scanning electron microscopy (SEM), plants were fixed in a solution of 1% glutaraldehyde in 50 mM sodium phosphate buffer for overnight, dehydrated in the ethanol series (30, 60, 80, 90, and 100%) and then transferred to a solution of isoamyl acetate. Samples were dried by a critical point dryer (JCPD-5, JEOL,
3
Testicular Histological Examination
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Testes were fixed with Bouin’s solution overnight, embedded in paraffin, and sectioned at 6 μm. After deparaffinisation, the sections were stained with haematoxylin and eosin according to standard procedures. Observations were performed under a microscope (AxioImager.M2; Carl Zeiss, Germany) equipped with a camera (Eos Kiss X2; Canon, Japan).
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