DNA coated beads were generated using kilobaseBINDER Dynabeads (Invitrogen). Non-coding DNA of the pCR2.1 plasmid (standard cloning plasmid from Life technologies TOPO TA cloning kit) was amplified by PCR. The forward primer contained a biotin attached to a linker (Biotin-TEG) at the 5′ end, while the reverse primer was labeled with Alexa Fluor 546 at the 5′ end. The resulting amplicon of 250 base pairs was gel-purified and 400 ng of the product were coupled to 5 μl kilobaseBINDER Dynabeads according to manufacturer’s instructions. Beads were then transferred to 100 μl assay buffer (20 mM Tris pH 7.5, 150 mM NaCl, 0.01% TX-100) and stored at 4°C.
Kilobasebinder dynabeads
Manufactured by Thermo Fisher Scientific
The KilobaseBINDER Dynabeads are magnetic beads designed for the rapid and efficient separation of DNA fragments ranging from 0.5 to 40 kilobases in length. They provide a versatile tool for DNA purification, size selection, and targeted enrichment applications in molecular biology research.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using kilobasebinder dynabeads
1
DNA-Bead Conjugation Protocol
2
Magnetic Bead-Based DNA Labeling Protocol
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