Mouse mpo elisa kit

Hemoglobin (Hb), myeloperoxidase (MPO), and interleukin (IL-6) content was measured by ELISA (Mouse Hemoglobin ELISA Kit, ab157715—Mouse MPO ELISA kit, ab155458; Abcam, Paris, France—Mouse IL-6 ELISA Kit, 88-7064-88, eBioscience, Santa Clara, USA) using brain homogenates prepared from TTC sections. Hb, MPO, and IL-6 concentration values were reported on total protein content from the ischemic hemisphere.

The mouse MPO ELISA kit was purchased from Abcam (Cat. No. ab275109), PYY ELISA kit from Aviva Systems Biology (Cat. No. OKCD05062, USA), mouse TNF-α and IL-6 ELISA kits from Biolegend (Cat. No. 431,301 and 430,901, USA). The protocols were performed as the manufacturers described. In brief, 96-well plates were coated with the corresponding capture antibody. After incubation with blocking buffer (PBS + 1% bovine serum albumin), a 100-µl aliquot of either standards, serum, or colonic protein extracts (5 mg tissue per mL PBS supplemented with protease inhibitors) was added into each well individually and the plates were incubated for 2 h at room temperature. The detector antibody was added to the wells, the plate was incubated, and unbound detector antibody was removed by washing. The avidin-peroxidase conjugate was added, the plate was incubated, and unbound conjugate was removed by washing. The enzymatic reaction catalyzed by horseradish peroxidase (HRP) was initiated by adding the substrate, 3,3’,5,5’ tetramethylbenzidine dihydrochloride (TMB). The absorbance was measured by using a microplate reader at a wavelength 450 nm after the reaction was stopped with 2 M H₂SO₄.

The concentrations of AMP, GMP, ADSS, and IMPDH2 in BM fluid of db/db mice were measured using an ELISA. The enzymatic activity of ADSS and IMPDH2 in lineage^−/low cells were also determined by ELISA, in accordance with the manufacturer’s instructions (MLBio, Shanghai, China). Plasma lipase and amylase activities were measured using commercial kits and quantified by a Tecan Safire microplate reader as previously described^{12 (link)}. Plasma levels of myeloperoxidase (MPO) were determined using a Mouse MPO ELISA Kit (Abcam, Shanghai, China).