Metoclopramide hydrochloride (MTC) was kindly gifted from Egyptian International Pharmaceutical Industries Company (EIPICO), Tenth of Ramadan City, Egypt. Potassium phosphotungstate was kindly supplied as a gift from National Research Center, Pharmaceutical Technology department, Cairo, Egypt. Cholesterol, Poly ethylene glycol 600 (PEG 600), Acetonitrile HPLC grade, and Chitosan of medium molecular weight (Sigma Aldrich, St. Louis, MO, USA). Tween 80 and Ethyl alcohol (99%) (El-Nasr Pharmaceutical Chemicals Co., Abuzaabal, Cairo, Egypt). Orthophosphoric acid and Span® 60 (Merck Schuchardit OHG, Hohenburnn, Germany). Carbopol 940, Acros Organics (Morris Plains, NJ, USA). Poloxamer 407 (Xi’an Lyphar Biotech Co., Ltd, Xi’an, China). Disodium hydrogen phosphate (Na2HPO4), Potassium dihydrogen orthophosphate (KH2PO4), and Sodium chloride (NaCl) (Oxford Lab Chem, Mumbai, India). Acetonitrile (Supelco, Bellefonte, PA, USA), Chloroform HPLC grade (Honeywell Riedel-de Haën, Seelze, Germany). HPLC grade water (Merck, Kenilworth, NJ, USA).
Poly ethylene glycol 600 peg 600
Manufactured by Merck Group
Sourced in United States
Polyethylene glycol 600 (PEG 600) is a clear, colorless, and odorless liquid. It is a polymer composed of repeating ethylene glycol units, with an average molecular weight of 600 Daltons. PEG 600 is commonly used as a solvent, plasticizer, and lubricant in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (3 mentions)
Chitosan, by Merck Group (2 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Carbopol 940, by Thermo Fisher Scientific (1 mentions)
Hplc grade water, by Merck Group (1 mentions)
Chloroform hplc grade, by Honeywell (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Anti ki67 antibody, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Bio Basic (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
2 iminothiolane hydrochloride, by Merck Group (1 mentions)
Clopidogrel, by Merck Group (1 mentions)
Tetrabutylammonium hydroxide, by Merck Group (1 mentions)
Ckx41 inverted fluorescence microscope, by Olympus (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
4 protocols using poly ethylene glycol 600 peg 600
1
Metoclopramide Nanoparticle Formulation
2
PHBV/PEG600 Hydrogel for Ranibizumab Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
PHBV with 3 wt% polyhydroxyvalerate (PHV) was supplied by Tianan Biologic Materials Co. Ltd. (Ningbo, China). Polyethylene glycol 600 (PEG600) and M199, as the cell culture media, were purchased from Sigma-Aldrich (St. Louis, USA). Dextran sulfate sodium (DS) from Leuconostoc spp. with a relative molecular mass (Mr) of 5000, as well as hydrochloric acid (HCl), was provided by Merck (Germany). Ranibizumab (Lucentis®, 10 mg/mL) was obtained from Genentech Inc. (CA, USA). Also, a microBCATM Protein Assay Kit and phosphate-buffered saline (PBS) tablets were purchased from BioBasic (Canada). The human umbilical vein endothelial cells (HUVECs) and fibroblast cell line were also supplied by Pasteur Institute of Iran. Finally, fetal bovine serum (FBS), penicillin, and streptomycin were provided by Invitrogen (Life Technologies, UK), and anti‐Ki67 antibody was supplied by Abcam (LA, USA).
+ Expand
PHBV with 3 wt% polyhydroxyvalerate (PHV) was supplied by Tianan Biologic Materials Co. Ltd. (Ningbo, China). Polyethylene glycol 600 (PEG600) and M199, as the cell culture media, were purchased from Sigma-Aldrich (St. Louis, USA). Dextran sulfate sodium (DS) from Leuconostoc spp. with a relative molecular mass (Mr) of 5000, as well as hydrochloric acid (HCl), was provided by Merck (Germany). Ranibizumab (Lucentis®, 10 mg/mL) was obtained from Genentech Inc. (CA, USA). Also, a microBCATM Protein Assay Kit and phosphate-buffered saline (PBS) tablets were purchased from BioBasic (Canada). The human umbilical vein endothelial cells (HUVECs) and fibroblast cell line were also supplied by Pasteur Institute of Iran. Finally, fetal bovine serum (FBS), penicillin, and streptomycin were provided by Invitrogen (Life Technologies, UK), and anti‐Ki67 antibody was supplied by Abcam (LA, USA).
3
Development of Chitosan-Based Drug Delivery System
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+ Expand
DH was provided from Jai Radhe Sales (Gujarat, India). Chitosan (103.2 g/mol, degree of deacetylation 76.6%), 2-imino thiolane hydrochloride, 2-mercaptoethanol, triethanolamin reagent grade, formic acid (ultra-performance liquid chromatography MS/MS (UPLC MS/MS) grade), clopidogrel (as internal standard (IS)), ammonium formate, and acetonitrile were purchased from Sigma Aldrich Company (St. Luis, MO, USA). Cholesterol (CHOL) and dipalmitoylphosphocholine (DPPC) were purchased from Fisher Scientific Company (Fair Lawn, NJ, USA) and Avanti Polar Lipid Inc., (Birmingham, AL, USA), respectively. Polyvinyl pyrolidone (PVP), known as Luviskol® K-9, was purchased from BASF (Aktien gesellschaft Ludwig Shafen, Germany). Polyethylene glycol 600 (PEG600) was obtained from Merck Schuchardt (Hohenbrunn, Germany). Lactic acid glutaraldehyde 50% solution and chloroform were purchased from BDHL laboratory Supplies (PooleBH151TD, England). Mono basic potassium dihydrogen phosphate and cellophane membrane (Spectra/Por® Dialysis Tubing from regenerated cellulose) with cut-off 12–14000 Da were purchased from panreac Quimica, SA, (Barcelona, Espana) and Spectrum Laboratories (Houston, TX), respectively. All chemicals used were of analytical grade and were used as received.
4
Comprehensive Characterization of Polymer-Based Biomaterials
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+ Expand
Chemical
and physical characterizations were performed using Shimadzu TGA-50,
Shimadzu DTA-50, Shimadzu DSC-60, Park Systems XE-100E AFM, LEO-EVO
40× SEM, Bruker NMR 300 MHz Ultrashield, Ascend 600 ULW, and
PerkinElmer UATR Two FTIR devices. All spectrophotometric analyses
were carried out with a Shimadzu UV-1601 UV–visible spectrophotometer
and BioTek Eon Elisa microplate reader. The adhesion results were
obtained with an MTS E42 test analyzer. L-929 cell morphologies were
determined by a JuliFL cell analyzer and an Olympus CKX41 inverted/fluorescence
microscope. Histological analysis was carried out with Leica DFC-280
microscope and Leica Q Win Image Analyze System (Leica Micros Imaging
Solutions Ltd., Cambridge, UK).
Polyethylene glycol 200 (PEG200),
polyethylene glycol 400 (PEG400), and polyethylene glycol 600 (PEG600)
were purchased from Merck. 4,4′-Methylenebis cyclohexyl diisocyanate,
β-cyclodextrin, silk sericin (10–40 kDa), toluene-4-sulfonyl
isocyanate, tetrabutylammonium hydroxide, MTT (thiazolyl blue tetrazolium
bromide), Dulbecco’s modified Eagle’s medium (DMEM),
gentamicin sulfate, and 2-isocyanatoethyl methacrylate were purchased
from Sigma-Aldrich. DMSO, acetonitrile, methanol, ethanol, and other
solvents were obtained from Merck. Irgacure-2959 was purchased from
TCI (Tokyo Chemical Industry). All chemicals were used without further
purification.
and physical characterizations were performed using Shimadzu TGA-50,
Shimadzu DTA-50, Shimadzu DSC-60, Park Systems XE-100E AFM, LEO-EVO
40× SEM, Bruker NMR 300 MHz Ultrashield, Ascend 600 ULW, and
PerkinElmer UATR Two FTIR devices. All spectrophotometric analyses
were carried out with a Shimadzu UV-1601 UV–visible spectrophotometer
and BioTek Eon Elisa microplate reader. The adhesion results were
obtained with an MTS E42 test analyzer. L-929 cell morphologies were
determined by a JuliFL cell analyzer and an Olympus CKX41 inverted/fluorescence
microscope. Histological analysis was carried out with Leica DFC-280
microscope and Leica Q Win Image Analyze System (Leica Micros Imaging
Solutions Ltd., Cambridge, UK).
Polyethylene glycol 200 (PEG200),
polyethylene glycol 400 (PEG400), and polyethylene glycol 600 (PEG600)
were purchased from Merck. 4,4′-Methylenebis cyclohexyl diisocyanate,
β-cyclodextrin, silk sericin (10–40 kDa), toluene-4-sulfonyl
isocyanate, tetrabutylammonium hydroxide, MTT (thiazolyl blue tetrazolium
bromide), Dulbecco’s modified Eagle’s medium (DMEM),
gentamicin sulfate, and 2-isocyanatoethyl methacrylate were purchased
from Sigma-Aldrich. DMSO, acetonitrile, methanol, ethanol, and other
solvents were obtained from Merck. Irgacure-2959 was purchased from
TCI (Tokyo Chemical Industry). All chemicals were used without further
purification.
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