Quantum gx software

S/Trp53^—/—, and S/P/E/Trp53^—/— LBSCs were injected orthotopically into the left lungs (final volume 50 ml) of 8~10 weeks old wild-type C57BL/6 mice (both male and female) using a 30-gauge needle. For IVIS and μCT imaging, mice were anaesthetized by isoflurane inhalation, administered 150 mg kg⁻¹ d-luciferin by intraperitoneal injection and placed in a mouse imaging shuttle device that maintains the sedated mouse in a stationary position. At 8 minutes after luciferin injection, bioluminescence was measured using a Perkin Elmer IVIS imaging system (Caliper Life Sciences-Xenogen, Hopkinton, MA). For μCT imaging, mice were scanned for 4 min under isoflurane anesthesia using a small animal Quantum FX μCT (PerkinElmer) at 45 μm resolution, 90 kV, with 160 μA current. Images were acquired using PerkinElmer Quantum GX software. The bioluminescence optical and CT datasets were co-registered in 3-D using the Living Image 4.5 software. Mouse lung tissues were prepared for histology and hematoxylin/eosin staining was per- formed as previously described (Regala et al., 2009 (link)). All animal experiments were performed under an approved IACUC protocol of Mayo Clinic.