Capsaicin, chloroquine, rapamycin, and the ULK1 inhibitor (SBI-0206965) were obtained from Sigma-Aldrich (St. Louis, MO, United States). MG132 was purchased from TOCRIS Bioscience (Ellisville, MO, United States). Specific antibodies against ribophorin II and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX, United States). Antibodies against poly (ADPribose) polymerase, caspase-3, phospho-elF2α, XBP1, ATG5, LC3B, phospho-MLKL (S358), and phospho-RIP3 (S227) were obtained from Cell Signaling Technology (Beverly, MA, United States). The antibody against p62 was purchased from Sigma-Aldrich. The antibody against phospho-IRE1α was obtained from Abcam (Cambridge, United Kingdom). The antibody against TRIB3 was obtained from Atlas Antibodies (Bromma, Sweden). The antibody against beclin 1 was purchased from Novus Biologicals (Centennial, CO, United States). The antibody against phospho-MLKL (T357/S358/S360) was purchased from ABclonal (Woburn, MA, United States).
Antibody against beclin 1
Manufactured by Novus Biologicals
Sourced in United States
Antibody against beclin 1 is a laboratory reagent used to detect and study the beclin 1 protein. Beclin 1 is a protein involved in the regulation of autophagy, a cellular process that recycles damaged or unwanted components. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunoprecipitation, to investigate the expression and localization of beclin 1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Capsaicin, by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Mg132, by Bio-Techne (1 mentions)
Sbi 0206965, by Merck Group (1 mentions)
Il 1β, by Merck Group (1 mentions)
P akt, by Abcam (1 mentions)
Ly294002, by Abcam (1 mentions)
P mtor, by Abcam (1 mentions)
Dylight 800 4x peg conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
P pi3k, by Abcam (1 mentions)
2 protocols using antibody against beclin 1
1
Autophagy and Necroptosis Regulation Assays
2
Regulation of Chondrocyte Autophagy
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PG545 was purchased from Progen Pharmaceuticals (Brisbane, Australia) and stored at -20°C [22] . IL-1β and 3-MA were purchased from Sigma-Aldrich (St Louis, MO, USA). A lentivirus expressing red fluorescent protein (RFP), green fluorescent protein (GFP), and LC3 were purchased from Pudong Genomeditech (Shanghai, China). LY294002 and antibodies against GADPH, cleaved-caspase 3, cleaved-PARP, collagenase type II (Col II), aggrecan, P62, cyclooxygenase-2 (COX-2), matrix metalloproteinase 13 (MMP13), PI3K, p-PI3K, Akt, p-Akt, mTOR, and p-mTOR were from Abcam (Cambridge, MA, USA). The antibody against Beclin-1 was from Novus Biologicals (Littleton, CO, USA). The antibody against LC3 and the DyLight™ 800 4X PEG-conjugated secondary antibody were from Cell Signaling Technology (Beverly, MA, USA).
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