Zonulin (Promocell Germany), BDG (Mybiosource Inc. CA), I-FABP, sCD14, sCD163, sTNFRI, hsCRP, IL-6 (R &D Systems, Minneapolis, Minnesota, USA) and oxidized LDL (ALPCO, Salem, New Hampshire, USA and Mercodia, Uppsala, Sweden) were measured by ELISA . The intra-assay variability ranged between 4-8% and inter-assay variability was less than 10% for all markers. All assays were done at Dr Funderburg’s laboratory at Ohio State University, Columbus, OH (https://u.osu.edu/caffre/people/nicholas-t-funderburg-ph-d/ ). Laboratory personnel were blinded to group assignments.
Stnfri
Manufactured by R&D Systems
Sourced in United States, Japan
STNFRI is a laboratory instrument designed for the detection and measurement of Soluble TNF Receptor I (sTNFRI) in various sample types. The core function of this product is to provide researchers with a reliable and quantitative tool for analyzing sTNFRI levels, which is important for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 6 (il 6), by R&D Systems (2 mentions)
Hscrp, by R&D Systems (1 mentions)
Zonulin, by PromoCell (1 mentions)
Scd163, by R&D Systems (1 mentions)
Scd14, by R&D Systems (1 mentions)
I fabp, by R&D Systems (1 mentions)
Traf2, by Cell Signaling Technology (1 mentions)
Stnf rii, by R&D Systems (1 mentions)
3 protocols using stnfri
1
Biomarker Quantification in Gut Inflammation
2
Antibodies and Reagents for TNF-RI Signaling
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Antibodies directed against soluble tumor necrosis factor receptor I (sTNF-RI), lymphotoxin-α (LTα or TNFSF1) were purchased from R&D systems. Antibodies recognizing phospho p65 (Ser536; P-p65), phospho IκBα (Ser32; P-IκBα), and TNF receptor-associated factor 2 (Traf2) were obtained from Cell Signaling Technology. Anti-fibronectin, RalA (Ras-related protein) and pan-actin were from Becton–Dickinson. Antibodies directed against actinin and collagen VI were purchased from Sigma and Biomol, respectively. Enbrel (Etanercept) and pentoxifylline (PTX) were obtained from Amgen and Sanofi-Aventis, respectively. The MIP-2α ELISA kit was purchased from R&D systems.
3
Evaluation of Inflammatory Markers in Serum
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The serum concentrations of IL-18, IL-6, neopterin, sTNF-RI, and sTNF-RII were evaluated using commercial enzyme-linked immunosorbent assays according to the manufacturer's instructions (IL-18: MBL, Nagoya, Japan; IL-6, sTNF-RI, and sTNF-RII: R&D Systems, Minneapolis, MN, USA; neopterin: IBL, Hamburg, Germany). neopterin, a 2-amino-4-hydroxy-(1′2′3′-trihydroxypropyl)-pteridine, is produced from guanosine triphosphate (GTP) via GTP cyclohydrolase I by activated monocytes/macrophages [26] . The activity of this enzyme is greatly enhanced by IFN-γ [26] . IFN-γ, which is released by activated type 1 helper T (Th1) lymphocytes and natural killer (NK) cells, is the most potent inducer of neopterin production. Serum TNF-α levels were undetectable. Instead, we measured serum sTNF-RI and -RII levels.
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