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Clone epr3864

Manufactured by Roche
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Clone EPR3864 is a laboratory equipment product. It is designed for use in research and laboratory settings. The core function of this product is to provide a platform for various experimental and analytical procedures. Further details on its intended use or specifications are not available.

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Lab products found in correlation

Clone mib 1, by Agilent Technologies (1 mentions) Clone umb1, by Abcam (1 mentions) Clone qbend 10, by Roche (1 mentions) Clone kp1, by Roche (1 mentions) Ventana discovery xt, by Roche (1 mentions) Bluing reagent, by Roche (1 mentions) Ultraview universal diaminobenzidine detection kit, by Roche (1 mentions) Hematoxylin 2, by Roche (1 mentions)

Close spelling variants of this product

ERG (clone EPR3864; EPR3864

4 protocols using clone epr3864

1

Immunohistochemical Staining of Tumor Markers

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The streptavidin–biotin peroxidase complex method was utilized for immunohistochemical studies with antibodies to epithelial membrane antigen (EMA; Clone E29, Ventana, monoclonal), somatostatin receptor 2 (SSTR2; Clone UMB1, Abcam, 1:500, monoclonal), smooth muscle actin (SMA; Clone 1A4, Cell Marque, 1:200 monoclonal), Ki-67 (MIB-1; Clone MIB-1, Dako,1:200 monoclonal), CD34 (Clone QBEnd-10, Ventana, monoclonal), ERG (Clone EPR3864, Ventana; monoclonal), CD68 (Clone KP1, Ventana, monoclonal). Antibodies were obtained and applied at ready-to-use dilutions unless stated otherwise.
Bale T.A., Benhamida J., Roychoudury S., Villafania L., Wrzolek M.A., Bouffard J.P., Bapat K., Ladanyi M, & Rosenblum M.K. (2020). Infarction with associated pseudosarcomatous changes mimics anaplasia in otherwise grade I meningiomas. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(7), 1298-1306.
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2

ERG Immunohistochemistry Protocol

Cited 2 times
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ERG protein immunohistochemistry was performed as previously described on standard histologic slides or tissue microarray spots using a rabbit monoclonal anti-ERG antibody (Clone EPR3864; Ventana Medical Systems) (23 (link)), and was scored dichotomously using a previously genetically-validated scoring system (24 (link)).
Lotan T.L., Kaur H.B., Salles D., Murali S., Schaeffer E.M., Lanchbury J.S., Isaacs W.B., Brown R., Richardson A.L., Cussenot O., Cancel-Tassin G., Timms K.M, & Antonarakis E.S. (2021). Homologous recombination deficiency (HRD) score in germline BRCA2- versus ATM-altered prostate cancer. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 34(6), 1185-1193.
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3

ERG Immunohistochemistry Protocol

Cited 2 times
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ERG protein immunohistochemistry was performed as previously described on standard histologic slides or tissue microarray spots using a rabbit monoclonal anti-ERG antibody (Clone EPR3864; Ventana Medical Systems) (23 (link)), and was scored dichotomously using a previously genetically-validated scoring system (24 (link)).
Lotan T.L., Kaur H.B., Salles D., Murali S., Schaeffer E.M., Lanchbury J.S., Isaacs W.B., Brown R., Richardson A.L., Cussenot O., Cancel-Tassin G., Timms K.M, & Antonarakis E.S. (2021). Homologous recombination deficiency (HRD) score in germline BRCA2- versus ATM-altered prostate cancer. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 34(6), 1185-1193.
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4

Automated Immunohistochemistry for Protein Detection

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Immunohistochemical detection of different proteins was performed using the Ventana Discovery XT automated staining system (Ventana Medical Systems, Tucson, AZ). Paraffin-embedded tissue microarray blocks were sectioned into 4-mm slices, deparaffinized, and antigens were demasked in EDTA buffer (pH 8.4). Primary antibodies used were specific for ERG (clone EPR3864, ready to use; Ventana Medical Systems, Tucson, AZ), EP1 to EP4 (all polyclonal rabbit Ig; all Cayman Chemical, Ann Arbor, MI), or IL-6 (polyclonal rabbit Ig; Abcam, Cambridge, MA). Secondary staining was conducted using a biotin-free detection kit (UltraMap anti-Rb Detection Kit). Color was developed using alkaline phosphatase (Ultra-Map anti-Rb Alk Phos; Ventana Medical Systems) for IL-6 or 3-3 0 -diaminobenzidine (UltraView Universal Diaminobenzidine Detection kit; Ventana Medical Systems) for all other antigens. Finally, slides were counterstained with Hematoxylin II and Bluing Reagent (Ventana Medical Systems).
Merz C., von Mässenhausen A., Queisser A., Vogel W., Andrén O., Kirfel J., Duensing S., Perner S, & Nowak M. (2016). IL-6 Overexpression in ERG-Positive Prostate Cancer Is Mediated by Prostaglandin Receptor EP2. The American journal of pathology, 186(4).
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