35 μm sections of labeled brains were stained for EGFP, and mounted on membrane PEN slides (Carl Zeiss). Labeled neurons were visualized, and captured into adhesive cap tubes (Carl Zeiss) using a PALM laser microdissection system (Carl Zeiss) using a uv laser to cut and flip tissue samples directly into inverted adhesive caps placed above slides. RNA was extracted using an RNeasy FFPE kit (QIAGEN). Reverse transcription was performed using random hexamers and Thermoscript reverse transcriptase (Fisher Scientific). Real time PCR was performed using SYBR® Green PCR Master Mix (Thermo Fisher) on a Realplex4 cycler (Eppendorf). We confirmed the specificity and size of the amplicons by running the PCR product on agarose gels, and by melting curve analysis. For Klhl14-overexpression experiments, data were analyzed using an unpaired two-sided t test. The PCR primers are listed in Key Resources Table .
Membrane pen slides
Manufactured by Zeiss
Membrane PEN slides are a type of laboratory equipment manufactured by Zeiss. They are designed for use in various scientific applications that require the handling and analysis of membrane samples. The core function of these slides is to provide a stable and controlled environment for the observation and study of membrane-based structures and processes.
Automatically generated - may contain errors
Lab products found in correlation
Sybr green pcr master mix, by Thermo Fisher Scientific (4 mentions)
Adhesivecap tube, by Zeiss (2 mentions)
P a l m laser microdissection system, by Zeiss (2 mentions)
Rneasy ffpe kit, by Qiagen (2 mentions)
Realplex4 cycler, by Eppendorf (2 mentions)
Thermoscript reverse transcriptase, by Thermo Fisher Scientific (2 mentions)
2 protocols using membrane pen slides
1
EGFP-labeled Neuron Transcriptome Analysis
2
EGFP-labeled Neuron Transcriptome Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
35 μm sections of labeled brains were stained for EGFP, and mounted on membrane PEN slides (Carl Zeiss). Labeled neurons were visualized, and captured into adhesive cap tubes (Carl Zeiss) using a PALM laser microdissection system (Carl Zeiss) using a uv laser to cut and flip tissue samples directly into inverted adhesive caps placed above slides. RNA was extracted using an RNeasy FFPE kit (QIAGEN). Reverse transcription was performed using random hexamers and Thermoscript reverse transcriptase (Fisher Scientific). Real time PCR was performed using SYBR® Green PCR Master Mix (Thermo Fisher) on a Realplex4 cycler (Eppendorf). We confirmed the specificity and size of the amplicons by running the PCR product on agarose gels, and by melting curve analysis. For Klhl14-overexpression experiments, data were analyzed using an unpaired two-sided t test. The PCR primers are listed in Key Resources Table .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!