Pcmvsport β galactosidase

Manufactured by InvivoGen

Sourced in United States

The PCMVsport β-galactosidase is a plasmid that contains the β-galactosidase gene under the control of the CMV promoter. This plasmid is commonly used as a reporter gene for monitoring gene expression in various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Passive lysis buffer (plb), by Promega (2 mentions) Porf9 mtnfα, by InvivoGen (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Deionized di water, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) N 1 2 3 dioleoyloxy propyl n n n trimethylammonium methyl sulfate dotap, by Roche (1 mentions) Endotoxin free giga plasmid kit, by Qiagen (1 mentions) Lactoferrin, by Merck Group (1 mentions) A431 human epidermoid carcinoma, by European Collection of Cell Cultures (1 mentions) Quanti it picogreen dsdna reagent, by Thermo Fisher Scientific (1 mentions) T98g human glioblastoma, by European Collection of Cell Cultures (1 mentions)

2 protocols using pcmvsport β galactosidase

Lactoferrin-Dendrimer Mediated Gold and Silver Nanoparticle Synthesis

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Lactoferrin (Lf), generation 3-diaminobutyric polypropylenimine (DAB) dendrimer, chloroauric acid (HAuCl₄), silver trifluoroacetate (CF₃COOAg), sodium hydrosulfide hydrate (NaSH), polyvinyl pyrrolidone (PVP, M_w 55,000 Da), diethylene glycol (DEG), sodium chloride (NaCl), aqueous hydrochloric acid solution (HCl, 37%), and deionized (DI) water (resistivity: 18.2 MΩ·cm) were obtained from Sigma Aldrich (Poole, UK). The expression plasmids encoding TNFα (pORF9-mTNFα) and β-galactosidase (pCMVsport β-galactosidase) were respectively purchased from InvivoGen (San Diego, USA) and Invitrogen (Paisley, UK). N-[1-(2,3-Dioleoyloxy) propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP) came from Roche (Burgess Hill, UK).
Passive lysis buffer was obtained from Promega (Southampton, UK). Label IT^® Fluorescein Nucleic Acid Labelling Kit and Vectashield^® mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI), respectively, came from Cambridge BioSciences (Cambridge, UK) and Vector Laboratories (Peterborough, UK). Bioware^® androgen-irresponsive PC-3M-luc-C6 human prostate adenocarcinoma expressing luciferase was obtained from Caliper Life Sciences (Hopkinton, MA). Minimum Modified Eagle's Medium (MEM), L-glutamine, fetal bovine serum (FBS) and penicillin-streptomycin were purchased from Life Technologies (Paisley, UK).

Almowalad J., Laskar P., Somani S., Meewan J., Tate R.J, & Dufès C. (2022). Lactoferrin- and Dendrimer-Bearing Gold Nanocages for Stimulus-Free DNA Delivery to Prostate Cancer Cells. International Journal of Nanomedicine, 17, 1409-1421.

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Transfection with Dendrimer and Cytokine Plasmids

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Lactoferrin and lactoferricin, generation 3-diaminobutyric polypropylenimine dendrimer (DAB) and the other chemicals were purchased from Sigma Aldrich (Poole, UK). The expression plasmids encoding Tumor necrosis factor (TNF) α (pORF9-mTNFα) and β-galactosidase (pCMVsport β-galactosidase) were obtained respectively from InvivoGen (San Diego, CA) and Invitrogen (Paisley, UK) and were purified using an Endotoxin-free Giga Plasmid Kit (Qiagen, Hilden, Germany). Passive lysis buffer was from Promega (Southampton, UK). Quanti-iT™ PicoGreen^® dsDNA reagent and tissue culture media were obtained from Invitrogen (Paisley, UK). Bioware^® B16-F10-luc-G5 mouse melanoma was obtained from Caliper Life Sciences (Hopkinton, MA). A431 human epidermoid carcinoma and T98G human glioblastoma were purchased from the European Collection of Cell Cultures (Salisbury, UK).

Lim L.Y., Koh P.Y., Somani S., Al Robaian M., Karim R., Yean Y.L., Mitchell J., Tate R.J., Edrada-Ebel R., Blatchford D.R., Mullin M, & Dufès C. (2015). Tumor regression following intravenous administration of lactoferrin- and lactoferricin-bearing dendriplexes. Nanomedicine, 11(6), 1445-1454.

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